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VEGF and bFGF mRNA Are Expressed in Ethylnitrosourea-Induced Experimental Rat Gliomas

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Abstract

1. Which angiogenic growth factors actually mediate tumor growth in ethylnitrosourea (ENU)-induced gliomas in rats was examined.

2. In situ hybridization histochemistry with digoxigenin-labeled oligonucleotide probes was used to investigate the cellular expression and distribution of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) mRNAs in ENU-induced gliomas.

3. Both VEGF and bFGF mRNAs were not detected in normal gial cells but in ENU-induced glioma cells.

4. Our results suggest that the growth of ENU-induced glioma may be regulated by multiple angiogenic growth factors and that these gliomas may proliferate by synthesizing such growth factors.

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Authors and Affiliations

  1. Department of Pathology, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki, 852, Japan

    Tomoaki Okimoto, Isao Shimokawa, Yoshikazu Higami & Takayoshi Ikeda

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  1. Tomoaki Okimoto
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  2. Isao Shimokawa
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  3. Yoshikazu Higami
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  4. Takayoshi Ikeda
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Okimoto, T., Shimokawa, I., Higami, Y. et al. VEGF and bFGF mRNA Are Expressed in Ethylnitrosourea-Induced Experimental Rat Gliomas. Cell Mol Neurobiol 17, 141–150 (1997). https://doi.org/10.1023/A:1026389306987

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