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Distribution of the Parathyroid Hormone-Related Peptide and Its Receptor in the Saccus Vasculosus and Choroid Plexus in the Red Stingray (Dasyatis akajei: Elasmobranch)

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Abstract

1. The exact role of the parathyroid hormone-related peptide (PTHrP) is not fully understood. We used immunohistochemistry to localize the PTHrP and its receptor in the brain of the red stingray, particularly in the saccus vasculosus (SV) and choroid plexus.

2. Immunoreactive PTHrP and its receptor were detected in the epithelial cells of the SV and the choroid plexus. In addition, the neuronal perikarya in the nucleus of the SV located in the hypothalamus is positive for the PTHrP.

3. No PTHrP-containing neurons were detected in the choroid plexus. Extracts of SV and choroid plexus showed positive reactions against the PTHrP and its receptor antibody in Western blot analysis.

4. High levels of immunoreactive PTHrP were detected in the plasma equivalent to those present in human humoral malignant hypercalcemia. In contrast, the immunoreactive PTHrP concentration in the cerebrospinal fluid was below detectable levels.

5. Our results suggest that the regulation of the PTHrP in the SV differs from that in the choroid plexus in the red stingray.

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Authors and Affiliations

  1. Department of Anatomy, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki, 852, Japan

    Kozo Akino, Tae Kawawaki & Junko Baba

  2. Department of Nature Medicine, Atomic Disease Institute, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki, 852, Japan

    Akira Ohtsuru, Yang Ting-Ting, Vera Braiden & Shunichi Yamashita

  3. Department of Molecular Pathology, Atomic Disease Institute, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki, 852, Japan

    Masahiro Nakashima, Masahiro Ito & Nobuharu Iwahori

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  1. Kozo Akino
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  2. Akira Ohtsuru
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  3. Masahiro Nakashima
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  4. Masahiro Ito
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  5. Yang Ting-Ting
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  6. Vera Braiden
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  7. Tae Kawawaki
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  8. Junko Baba
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  9. Shunichi Yamashita
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  10. Nobuharu Iwahori
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Akino, K., Ohtsuru, A., Nakashima, M. et al. Distribution of the Parathyroid Hormone-Related Peptide and Its Receptor in the Saccus Vasculosus and Choroid Plexus in the Red Stingray (Dasyatis akajei: Elasmobranch). Cell Mol Neurobiol 18, 361–368 (1998). https://doi.org/10.1023/A:1022509300758

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