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Stress Protein Inductions After Brain Ischemia

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Abstract

1. Hippocampal CA1 neurons are the most vulnerable to transient cerebral ischemia. However, the mechanism has not been fully understood.

2. The mRNAs for 72-kd (HSP72) and 73-kd (HSC73) heat shock proteins (HSPs), which are located mainly in the cytoplasm, were greatly induced together in CA1 cells, with a peak at 1–2 days in gerbils. However, immunoreactive HSP72 protein was only minimally expressed in CA1 neurons.

3. The mRNA for mitochondrial HSP60 began to increase at 3 hr in CA1 cells and was sustained until 1 day.

4. The level of mRNA for cytochrome c oxidase subunit I (COX-I) progressively decreased in CA1 neurons after a transient ischemia and completely disappeared at 7 days. The activity of cytochrome c oxidase (COX) protein also showed an early decrease in CA1 cells and was followed by a reduction in the level of COX-I DNA after 2 days.

5. These results suggest that HSP gene inductions were inhibited at the translational level but that mitochondrial DNA expression was disturbed at the transcriptional level. A disturbance of mitochondrial DNA expression could cause progressive failure of energy production of CA1 cells that eventually results in neuronal cell death.

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Author notes

  1. K. Abe

    Present address: Department of Neurology, Okayama University Medical School, 2-5-1 Shikatacho, Okayama, 700-8558, Japan

Authors and Affiliations

  1. Department of Neurology, Tohoku University School of Medicine, 1–1 Seiryo-machi, Aobaku, Sendai, 980, Japan

    K. Abe, J. Kawagoe, M. Aoki, K. Kogure & Y. Itoyama

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  4. K. Kogure
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  5. Y. Itoyama
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Abe, K., Kawagoe, J., Aoki, M. et al. Stress Protein Inductions After Brain Ischemia. Cell Mol Neurobiol 18, 709–719 (1998). https://doi.org/10.1023/A:1020694205003

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