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EGF and TGF-β1 Gene Expression in Chronically Rejecting Small Bowel Transplants

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Abstract

Long-term survival of small bowel transplants ishampered by chronic rejection. Epidermal growth factor(EGF) and transforming growth factor β (TGF-β)have opposing, regulatory roles in normal intestinal physiology and may be involved in thepathogenesis of chronic intestinal rejection. Our aimwas to investigate the expression of EGF andTGF-β1 in chronically rejecting smallbowel transplants. Orthotopic small bowel transplantation was performed inthe allogeneic DA-to-AS rat combination; Cyclosporin wasadministered temporarily to prevent acute rejection.Controls were DA isografts and normal DA rats. PreproEGF and TGF-β1 geneexpression was evaluated by northern blot analysis ofthe ileum RNA and standardized againstglyceraldehyde-3-phosphate-dehydrogenase expression.Allografts demonstrated functional impairment and histological features of chronicrejection, whereas isografts appeared normal. Allograftsdemonstrated a significant reduction of EGF mRNA whencompared to DA isografts. No significant changes were detected in TGF-β1expression in either allogeneic or syngeneic grafts. Inconclusion, this study demonstrates reduced preproEGFand preserved TGF-β1 gene expression inchronically rejecting small bowel transplants.

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Authors
  1. Ewout A. Kouwenhoven
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  2. Alicia N. Stein-Oakley
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  3. Paula Jablonski
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  4. Ron W. F. De Bruin
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  5. Napier M. Thomson
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Kouwenhoven, E.A., Stein-Oakley, A.N., Jablonski, P. et al. EGF and TGF-β1 Gene Expression in Chronically Rejecting Small Bowel Transplants. Dig Dis Sci 44, 1117–1123 (1999). https://doi.org/10.1023/A:1026659720191

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