Skip to main content
SpringerLink
Log in

Levels of messenger ribonucleic acids for plasma proteins in rat liver during acute experimental inflammation

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

The levels of mRNA for plasma proteins and for metallothionein in rat liver during the acute-phase response were studied by hybridization to specific cDNA probes. The mRNA forα 2 -macroglobulin, theβ-chain of fibrinogen, α1,-acid glycoprotein (so-called acute-phase reactants) reached a maximum level between 18 and 36 h after inducing an acute inflammation. The level of mRNA for metallothionein-I peaked earlier, after 12 h. The mRNA for transferrin showed a delayed increase with a broad maximum for its relative level after 36–60 h. The mRNA levels for albumin and α2u-globulin (so-called negative acute-phase reactants) decreased, reaching a minimum of 25 % of the normal level after 36 h (albumin) and after 72 h (α2u-globulin). The ratios of the rates of incorporation of leucine into the proteins over the levels of their mRNA in liver changed only a little, indicating that the rates of synthesis of plasma proteins in the liver are regulated at the mRNA level during the acute-phase response to inflammation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Mercer, J. F. B., andP. Hudson. 1982. Cloning of metallothionein cDNA from neonatal rat liver.Biosci Rep. 2:761–768.

    Google Scholar 

  2. Aldred, A. R., G. J. Howlett, andG. Schreiber. 1984. Synthesis of rat transferrin inEscherichia coli containing a recombinant bacteriophage.Biochem. Biophys. Res. Commun. 122:960–965.

    Google Scholar 

  3. Birch, H. E., M. Nagashima, R. J. Simpson, andG. Schreiber. 1983. Expression of the cDNA for α1,-acid glycoprotein, a rat plasma protein, inEscherichia coli. Biochem. Int. 6:653–661.

    Google Scholar 

  4. Northemann, W., M. Heisig, D. Künz, andP. C. Heinrich. 1985. Molecular cloning of cDNA sequences for rat α2-macroglobulin and measurement of its transcription during experimental inflammation.J. Biol. Chem. 260:6200–6205.

    Google Scholar 

  5. de Jong, F. A., G. Howlett, A. R. Aldred, N. Fidge, andG. Schreiber. 1984. Synthesis of rat apolipoprotein E byEscherichia coli infected with recombinant bacteriophage.Biochem. Biophys. Res. Commun. 119:657–662.

    Google Scholar 

  6. Dickson, P. W. 1985. Rat transthyretin: Biosynthesis and metabolism. PhD thesis. University of Melbourne.

  7. Schrieber, G., G. Howlett, M. Nagashima, A. Millership, H. Martin, J. Urban, andL. Kotler. 1982. The acute-phase response of plasma protein synthesis during experimental inflammation.J. Biol. Chem. 257:10271–10277.

    Google Scholar 

  8. Schreiber, G., andG. Howlett. 1983. Acute-phase proteins.In Plasma Protein Secretion by the Liver. H. Glaumann, T. Peters Jr., and C. Redman, editors. Academic Press, New York. 423–449.

    Google Scholar 

  9. Maniatis, T., E. F. Fritsch, andJ. Sambrook. 1982. Molecular Cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

    Google Scholar 

  10. White, B. A., andF. C. Bancroft. 1982. Cytoplasmic dot hybridization.J. Biol. Chem. 257:8569–8572.

    Google Scholar 

  11. Thomas, P. S. 1980. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proc. Natl. Acad. Sci. U.S.A. 77:5201–5205.

    Google Scholar 

  12. Cole, T., A. Inglis, M. Nagashima, andG. Schreiber. 1985. Major acute-phase alpha(1)protein in the rat: Structure, molecular cloning, and regulation of mRNA levels.Biochem. Biophys. Res. Commun. 126:719–724.

    Google Scholar 

  13. Cole, T., A. S. Inglis, C. M. Roxburgh, G. J. Howlett, andG. Schreiber. 1985. Major acute-phaseα 1 -protein of the rat is homologous to bovine kininogen and contains the sequence for bradykinin: Its synthesis is regulated at the mRNA level. FEBS Lett.183:57–61.

    Google Scholar 

  14. Dickson, P. W., G. J. Howlett, andG. Schreiber. 1985. Rat transthyretin (prealbumin): Molecular cloning, nucleotide sequence, and gene expression in liver and brain.J. Biol. Chem. 260:8214–8219.

    Google Scholar 

  15. Ricca, G. A., R. W. Hamilton, J. W. McLean, A. Conn, J. E. Kalinyak, andJ. M. Taylor. 1981. Ratα 1 ,-acid glycoprotein mRNA.J. Biol. Chem. 256:10362–10368.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Russell Grimwade School of Biochemistry, University of Melbourne, 3052, Parkville, Victoria, Australia

    Gerhard Schreiber, Angela R. Aldred, Tim Thomas, Helen E. Birch, Phillip W. Dickson, Tu Guo-Fen, Geoffrey J. Howlett, Felice A. De Jong & Alana Mitchell

  2. Biochemisches Institut, Universität Freiburg, i.Br., Freiburg, Federal Republic of Germany

    Peter C. Heinrich & Wolfgang Northemann

Authors
  1. Gerhard Schreiber
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Angela R. Aldred
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tim Thomas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Helen E. Birch
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Phillip W. Dickson
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tu Guo-Fen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Peter C. Heinrich
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Wolfgang Northemann
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Geoffrey J. Howlett
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Felice A. De Jong
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Alana Mitchell
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schreiber, G., Aldred, A.R., Thomas, T. et al. Levels of messenger ribonucleic acids for plasma proteins in rat liver during acute experimental inflammation. Inflammation 10, 59–66 (1986). https://doi.org/10.1007/BF00916041

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00916041

Keywords

Search

Navigation