Abstract
The inflammatory response, antigen retention, and antigen localization was studied in mice after immunization with influenza virus glycoproteins presented in two physically defined forms-micelles and ISCOMS (immunostimulating complexes). Two hours after intraperitoneal injection, the proportion of polymorphonuclear leukocytes (PMNs) in peritoneal lavage cells increased from less than 1% to 82% in ISCOM-treated mice and from <1% to 41% of the total cell count in micelle-treated mice. For both treatment groups, the proportion of PMNs returned to around zero 24 h postimmunization. Total recovery of radioactive antigen was significantly greater (P<0.05) in ISCOM-treated than in micelle-treated mice at one, two, and eight days postinjection. At all times tested, animals given ISCOMs had significantly more radioactive antigen in their spleens than animals given micelles. By electron microscopy ISCOMs were found to attach externally to the plasma membrane or within phagosomes of macrophages in close association with the membranes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Colditz, I. G., R. L. Kerlin, andD. L. Watson. 1987. Migration of neutrophils and their role in elaboration of host defence.In Migration and Homing of Lymphoid Cells. A. J. Husband, editor. CRC Press, Boca Raton, Florida, Chapter 8.
Unanue, E. R., D. I., Beller, C. L. Lu, andP. M. Allen. 1984. Antigen presentation: Comments on its regulation and mechanism.J. Immunol. 132:1–5.
Karch, H., andK. Nixdorf. 1983. Modulation of the IgG subclass response to lipopolysaccharide by bacterial membrane components: Differential adjuvant effects produced by primary and secondary stimulation.J. Immunol. 131:6–8.
Morein, B., B. Sundquist, S. Höglund, K. Dalsgaard, andA. Osterhaus. 1984. Iscom, a novel structure for antigenic presentation of membrane proteins from enveloped viruses.Nature 308:457–460.
Morein, B., K. Lövgren, S. Höglund, andB. Sundquist. 1987. Iscom-an immunostimulating complex.Immunol. Today 8:333–338.
Höglund, S., K. Dalsgaard, K. Lövgren, B. Sundquist, A. Osterhaus, andB. Morein. 1989. ISCOMs and immunostimulation with viral antigens.Subcell. Biochem. 15:35–64.
LÖvgren, K., J. Lindmark, R. Pipkorn, andB. Morein. 1987. Antigenic presentation of small molecules and peptides conjugated to a preformed iscom as carrier.J. Immunol. Methods 98:137–143.
Yoffey, J. M., andF. C. Courtice. 1970. Lymph flows from regional lymphatics.In Lymphatics, Lymph and the Lymphomyeloid Complex. Academic Press, London.
Landsteiner, K., andJ. Jacobs. 1935. Studies on the sensitization of animals with simple chemical compounds.J. Exp. Med. 61:643–656.
Vischer, I. L., V. Bretz, andM. Baggiolini. 1976. In vitro stimulation of lymphocytes by neutral proteinases from human polymorphonuclear leucocyte granules.J. Exp. Med. 144:863–872.
Beh, K. J., andA. K. Lascelles. 1985. The effect of adjuvants and prior immunization on the rate and mode of uptake of antigen into efferent popliteal lymph from sheep.Immunology 54:487–495.
Morris, B. 1985.In Immunology of the Sheep. B. Morris and M. Miyasaka, editors. Editiones Roche, Basle, Switzerland, 440.
Humphrey, J. H. 1982. The fate of antigens.In Clinical Aspects of Immunology, 4th ed. P. J. Lachmann and D. K. Peters, editors. Blackwell Scientific, Oxford, 161.
Scott, M. J., M. Goss-Sampson, andR. Bomford. 1985. Adjuvant activity of saponin: Antigen localization studies.Int. Arch. Allergy Appl. Immunol. 77:409–412.
Lascelles, A. K., G.Eagleson, K. J.Beh, and D. L.Watson. 1989. Significance of Freund's adjuvant/antigen injection granuloma in the maintenance of serum antibody response.Vet. Immunol. Immunopathol. (in press).
Unanue, E. R., andB. A. Askonas. 1968. Persistence of immunogenicity of antigen after uptake by macrophages.J. Exp. Med. 127:915–926.
Murphy, P. 1976. Phagocytosis.In The Neutrophil. Plenum Medical, New York.
Hall, J. G., andD. Robertson. 1983. Phagocytosis in vivo of immune complexes by dendritic cells in the lymph of sheep.Int. Arch. Allergy Appl. Immunol. 73:155–161.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Watson, D.L., Lövgren, K., Watson, N.A. et al. Inflammatory response and antigen localization following immunization with influenza virus ISCOMs. Inflammation 13, 641–649 (1989). https://doi.org/10.1007/BF00914308
Issue Date:
DOI: https://doi.org/10.1007/BF00914308