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Role of resident macrophages in canatoxin-induced in vivo neutrophil migration

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Abstract

Canatoxin (Cntx), a toxic protein purified fromCanavalia ensiformis seeds, was shown to have lipoxygenase-mediated effects either in vivo or in vitro. Data here show that Cntx induced a dose-dependent migration of neutrophils and mononuclear cells when injected into rat peritoneal cavities. Furthermore, Cntx was able to induce neutrophil migration into pleural cavities and into air pouches. These effects were inhibited by dexamethasone but not by inhibitors of arachidonic acid metabolism (indomethacin, NDGA, and BW-755c) or by a PAF antagonist (BN 52021). In the peritoneal cavity Cntx caused an increase in vascular permeability inhibited by dexamethasone and BW-755c. Neutrophil migration induced by this toxin was dependent on the number of resident macrophages, since the migratory effect was enhanced by increasing the peritoneal macrophage population with thioglycollate pretreatmen; and was diminished when this population was reduced by peritoneal wash. It was also observed that Cntx induced release of a chemotactic factor from macrophage monolayers in vitro. Dexamethasone blocked this release but did not affect in vivo neutrophil recruitment induced by that factor. These data suggest that Cntx-induced neutrophil migration may be mediated by the same macrophage-derived neutrophil chemotactic factor released by other stimuli such as LPS, IL-1, and INF-gamma.

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Authors and Affiliations

  1. Department of Pharmacology Institute of Biology, Universidade do Estado do Rio de Janeiro, 20551, Rio de Janeiro, RJ, Brazil

    C. Barja-Fidalgo

  2. Department of Biochemistry Institute of Biological Sciences, Universidade Federal do Rio de Janeiro, 21910, Rio de Janeiro, Brazil

    C. Barja-Fidalgo, C. R. Carlini & J. A. Guimarães

  3. Department of Pharmacology of the Faculty of Medicine of Ribeirão Preto, University of São Paulo, 14049, Ribeiräo Preto, SP, Brazil

    C. A. Flores, F. Q. Cunha & S. H. Ferreira

Authors
  1. C. Barja-Fidalgo
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  2. C. R. Carlini
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  3. J. A. Guimarães
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  4. C. A. Flores
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  5. F. Q. Cunha
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  6. S. H. Ferreira
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Barja-Fidalgo, C., Carlini, C.R., Guimarães, J.A. et al. Role of resident macrophages in canatoxin-induced in vivo neutrophil migration. Inflammation 16, 1–12 (1992). https://doi.org/10.1007/BF00917510

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