Abstract
Purpose: This study was designed to examine whether hyaluronicacid (HA) inhibits apoptosis in cumulus and muralgranulosa cells and to examine whether this effect of HAwas mediated through CD44.
Methods: Mural and cumulus granulosa cells were obtainedfrom in vitro fertilization patients. The cells were culturedwith various concentrations of HA or HA plus variousconcentrations of anti-CD44 antibody without serum supplement.After 24 hr of culture, the cells were fixed and stainedwith Hoechst 33258. One thousand granulosa cells of eachconditions were observed by fluorescence microscopy.
Results: HA inhibited apoptosis in both kinds of granulosacells, and anti-CD44 antibody prevented this effect of HA.Conclusions: The incidence of apoptotic granulosa cellswith fragmented condensed nuclei was reduced by HA viaCD44.
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Kaneko, T., Saito, H., Toya, M. et al. Hyaluronic Acid Inhibits Apoptosis in Granulosa Cells via CD44. J Assist Reprod Genet 17, 162–167 (2000). https://doi.org/10.1023/A:1009470206468
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DOI: https://doi.org/10.1023/A:1009470206468