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Differential allelochemical detoxification mechanism in tissue cultures ofAntennaria microphylla andEuphorbia esula

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Abstract

Callus and suspension cultures ofAntennaria microphylla (small everlasting) and the noxious weedEuphorbia esula (leafy spurge) can glucosylate benzene-1,4-diol (hydroquinone) to the corresponding monoglucoside, arbutin. HPLC analysis of extracts from callus tissue corroborates the presence of hydroquinone in the cells of small everlasting. Constitutive levels of a UDPG-dependent glucosyltransferase were detected in cell-free extracts of this tissue. Although this detoxification enzyme was induced in leafy spurge suspension culture cells grown in the presence of hydroquinone, the activity was six-fold lower than that measured in small everlasting. Differential ability to detoxify hydroquinone provides a basis for the observed allelopathic interaction between small everlasting and leafy spurge.

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Authors and Affiliations

  1. U.S. Department of Agriculture, Agricultural Research Service Western Regional Research Center, 800 Buchanan Street, 94710, Albany, California

    Mary Ellen Hogan & Gary D. Manners

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  1. Mary Ellen Hogan
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  2. Gary D. Manners
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Hogan, M.E., Manners, G.D. Differential allelochemical detoxification mechanism in tissue cultures ofAntennaria microphylla andEuphorbia esula . Journal of Chemical Ecology 17, 167–174 (1991). https://doi.org/10.1007/BF00994430

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  • DOI: https://doi.org/10.1007/BF00994430

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