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Unmodified calcium concentrations in tumour necrosis factor receptor subtype-mediated apoptotic cell death

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Abstract

Tumour necrosis factor-α (TNF) receptors mediate a variety of effects dependent on cell type. A role for Ca2+ in TNF-induced death remains uncertain. Here we investigated restricting intracellular/extracellular Ca2+ in HeLa epithelial carcinoma cells expressing low and high levels of p75TNFR receptor subtype and KYM-1 rhabdomyosarcoma cells, models of rapid TNF-induced apoptosis. Ca2+-chelators EGTA and BAPTA-AM as well as microsomal Ca2+-ATPase inhibitor thapsigargin, did not alter TNF-induced death. TNF was also unable to alter resting [Ca2+]i levels which remained < 200 nM even during times when these cells were undergoing apoptotic cell death. These findings indicate no role for modulated Ca2+ concentrations in TNF-induced apoptotic cell death.

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Authors and Affiliations

  1. Department of Biomedical Sciences, Institute of Medical Sciences, Foresterhill, University of Aberdeen, Aberdeen, AB25 2ZD, UK

    Shona M. McFarlane, Helen M. Anderson, Steven J. Tucker, Orla J. Jupp & David J. McEwan

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  1. Shona M. McFarlane
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  2. Helen M. Anderson
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  3. Steven J. Tucker
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  4. Orla J. Jupp
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  5. David J. McEwan
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McFarlane, S.M., Anderson, H.M., Tucker, S.J. et al. Unmodified calcium concentrations in tumour necrosis factor receptor subtype-mediated apoptotic cell death. Mol Cell Biochem 211, 19–26 (2000). https://doi.org/10.1023/A:1007189911897

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