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“Calcium metabolism in intact isolated thymocytes”

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Summary

Isolated rat thymocytes incubated under proper metabolic conditions extrude Ca24 previously taken up under metabolically unfavourable conditions.

The extrusion can be supported by both respiratory and glycolytic energy but glycolysis seems to be more efficient for this purpose.

La3− (50–200 μM) and the ionophore A 23187 inhibit cell Ca2+ extrusion.

Ruthenium Red (1–100 μM)) does not influence cell Ca2+ extrusion while it inhibits the in situ mitochondrial cation uptake.

All the results are consistent with a cell regulation model of Ca 2+ content in which both plasma membrane and mitochondria co-operate, acting in opposite directions, in order to decrease cytosolic Ca 2+ concentration.

The possibility of Na+-Ca2+ hetero-exchange participation to cell Ca2+ homeostasis regulation is also discussed.

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Authors and Affiliations

  1. Institute of General Pathology, Catholic University, School of Medicine, Roma, Italy

    A. M. Dani, A. Cittadini, G. Calviello, G. Festuccia & T. Terranova

Authors
  1. A. M. Dani
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  2. A. Cittadini
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  3. G. Calviello
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  4. G. Festuccia
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  5. T. Terranova
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Dani, A.M., Cittadini, A., Calviello, G. et al. “Calcium metabolism in intact isolated thymocytes”. Mol Cell Biochem 22, 139–146 (1978). https://doi.org/10.1007/BF00496239

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