Summary
Practical peptide separation methods on reversed-phase high-performance liquid chromatography are reviewed with the description of our recent results. The retention mechanism of peptides on reversed-phase columns is discussed and the method used to estimate the retention time of peptides under some restricted conditions is presented.
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Imoto, T., Yamada, H. Peptide separation by reversed-phase high-performance liquid chromatography. Mol Cell Biochem 51, 111–121 (1983). https://doi.org/10.1007/BF00230396
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DOI: https://doi.org/10.1007/BF00230396