Abstract
Rats were raised in the absence of vitamin D in utero and throughout post-fetal life and neither 1,25-dihydroxyvitamin D3 nor related metabolites were detected in serums. No changes were observed in the relative amount of extractable noncollagenous bone proteins (NCP) in rachitic compared to vitamin-D-repleted animals. As expected, the relative levels of the mineral-bound, serum-derived albumin and α2-HS glycoprotein were unaffected in bones of rachitic animals. Interestingly, the vitamin D deficiency also did not have dramatic effects on several bone cell-derived noncollagenous proteins including: bone proteoglycans I & 11, bone sialoprotein li osteonectin, and osteocalcin. In contrast to the proteoglycans, the bone sialoprotein II and osteonectin were found in the nonmineral compartment of the rachitic animals, presumably bound to the wide osteoid seam.
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Gay S, Miller EJ: Collagen in the Physiology and Pathology of Connective Tissue, Gustav-Fisher Verlag, Stuttgart, 1978
Avioli IV: Collagen abnormalities and clinical bone disease. In: Veis A (ed) The Chemistry and Biology of Mineralized Connective Tissues. Elsevier/North-Holland, New York, 1981, pp 175–183
Hauschka PV, Lian JB, Gallop PM: Proc Natl Acad Sci USA 72:3925–3929, 1975
Price PA, Otsuka AS, Poser JW, Kristaponis J, Raman N: Characterization of γ-carboxyglutamic acid-containing protein from bone, Proc Natl Acad Sci USA 73:1447–1451, 1976
Lian JB, Glimcher MJ, Roufosse AH, Hauschka PV, Gallop PM, Cohen-Solal L, Reif B: Alterations of the γ-carboxyglutamic acid and osteocalcin concentration in vitamin D-deficient chick bone. J Biol Chem 257:4999–5003, 1972
Price PS, Baukol SA: 1,25 dihydroxyvitamin D3 increases synthesis of the vitamin K-dependent bone protein by osteosarcoma cells. J Biol Chem 255:11660–11663, 1980
Price PA, Baukol SA: 1,25 dihydroxyvitamin D3 increases serum levels of the vitamin K-dependent bone protein. Biochem Biophys Res Commun 99:928–935, 1981.
Termine JD, Belcourt AB, Conn KM, Kleinman HK: Mineral and collagen-binding proteins of fetal calf bone. J Biol Chem 256:10403–10408, 1981
Termine JD, Kleiman HK, Whitson SW, Conn KM, McGarvey ML, Martin GR: Osteonectin, a bone specific protein linking mineral to collagen. Cell 26:99–105, 1981
Fisher LW, Whitson SW, Avioli LV, Termine JD: Matrix sialoprotein of developing bone. J Biol Chem 258:12723–12727, 1983
Fisher LW, Termine JD, Dejter SW, Yanagishita M, Kimura JH, Hascall VC, Kleinman HK, Hassell JR, Nilsson B: Proteoglycans of developing bone. J Biol Chem 258:6588–6594, 1983
Fisher LW: The nature of the proteoglycans of bone. In: Butler WT (ed) The Chemistry and Biology of Mineralized Tissues. Ebsco Media, Birmingham Alabama, 1985. pp 188–196
Weintroub S, Hagan MP, Reddi AH: Reduction of hematopoietic stem cells and adaptive increase in cell cycle rate in rickets. Am J Physiol 243:C303-C306, 1982
Willis JB: The determination of metals in blood serum by atomic absorption spectroscopy. I Calcium Spectrochim Acta 16:259–272, 1960
Chen PS, Toribara TY, Warner H: Microdetermination of phosphorus. Anal Chem 28:1756–1759, 1956
Weisman Y, Reiter E, Root A: Measurement of 24, 25 dihydroxyvitamin D in sera of neonates and children. J Pediatr 91:904–908, 1977
Eisman JA, Hamstra AJ, Kream BE, DeLuca HF: A sensitive and convenient method for determination of 1,25-dihydroxyvitamin D in human plasma. Arch Biochem Biophys 176:235–243, 1976
Eisman JA, Shepard RM, Deluca HF: Determination of 25-hydroxyvitamin D3 in human plasma using highpressure liquied chromatography. Anal Biochem 80:298–305, 1977
Termine JD, Belcourt AB, Christner PJ, Conn KM, Nylen MU: Properties of dissociatively extracted fetal tooth matrix proteins. I Principal molecular species in developing bovine enamel. J Biol Chem 255:9760–9768, 1980
Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proc Natl Acad Sci USA 76:4350–4354, 1979
Termine JD: Chemical characterization of fetal bone matrix constituents. In: Veis A (ed) The Chemistry and Biology of Mineralized Connective Tissues. Elsevier/North-Holland, New York, 1981, pp 349–353
Termine JD: Osteonectin and other newly described proteins of developing bone. In: Peck WA (ed) Bone and Mineral Research. Annual 1. Excerpta Medica, Amsterdam, 1983, pp 144–156
Ashton BA, Triffitt JT, Herring GM: The isolation and partial characterization of two glycoproteins from bovine cortical bone. Eur J Biochem 45:525–533, 1974
Ashton BA, Hohling HJ, Triffitt JT: Plasma proteins present in human cortical bone: enrichment of the α2HS-glycoprotein. Calcif Tissue Res 22:27–33, 1976
Triffitt JT, Gebauer U, Ashton BA, Owen ME, Reynolds JJ: Origin of plasma α2HS-glycoprotein and its accumulation in bone. Nature (Lond.) 262:226–227, 1976
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Wientroub, S., Fisher, L.W., Reddi, A.H. et al. Noncollagenous bone proteins in experimental rickets in the rat. Mol Cell Biochem 74, 157–162 (1987). https://doi.org/10.1007/BF00224953
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DOI: https://doi.org/10.1007/BF00224953