Summary
A soluble lipid-protein complex in bovine retinal pigment epithelium is shown to carry endogenous 11-cis retinaldehyde, in the extent of 15% of the total 11-cis retinaldehyde found in this tissue. The complex, analyzed with respect to its chemical composition, exhibits a lipid composition close resembling the lipid composition of the rod outer segment membrane; the SDS-PAGE evidences the presence of a number of protein bands, two of which of 34 and 27 kDa appear glycoproteins. Finally, the lipid-protein complex exhibits a discrete level of a Cathepsin D-like protease activity. From the above, the possibility is discussed that the soluble lipid-protein complex could represent some phagolysosomal inclusion occurring in the pigment epithelial cells upon rod outer segment phagocytosis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Saari JC, Bredberg L, Garwin GG: Identification of endogenous retinoids associated with three cellular retinoid-binding proteins from bovine retina and retinal pigment epithelium. J Biol Chem 257:13329–13333, 1982
Stubbs GW, Saari JC, Futterman S: 11-cis retinal binding protein from bovine retina. J Biol Chem 254:8529–8533, 1979
Livrea MA, Bongiorno A, Tesoriere L, Nicotra C, Bono A: Binding of 11-cis retinaldehyde to the partially purified cellular retinaldehyde binding protein from bovine retinal pigment epithelium. Experientia 43:582–585, 1987
Futterman S, Saari JC, Blair S: Occurrence of a binding protein for 11-cis retinal in retina. J Biol Chem 252:3267–3271, 1977
Groenendijk GWT, Jansen PAA, Bonting SL, Daemen FJM: Analysis of geometrically isomeric vitamin A compounds. In: McCormick DB and Wright LD (eds), Methods in Enzymol. Academic Press, New York, 1980, vol 67, pp 203–220
Feeney-Burns L, Berman ER: Methods for isolating and fractionating pigment ephitelial cells. In: Packer, L (ed) Methods in Enzymol. Academic Press, New York, 1982, vol 81, pp 95–110
Rothmans JP, Kropf A: The analysis of retinal isomers by high speed liquid chromatography. Vision Res 15:1301–1302, 1975
Dittmer JC, Wells MA: Quantitative and qualitative analysis of lipids and lipid components. In: Lowenstein, JM (ed) Methods in Enzymol. Academic Press, New York, 1969, vol 14, pp 482–530
Bobbit JM: Thin Layer Chromatography, Reinhold Publishing Corporation, New York, 1964, p 124
Skipski VP, Barclay M: Thin-layer chromatography of lipids. In: Lowenstein, JM (ed) Methods in Enzymol. Academic Press, New York, 1969, vol 14, pp 530–598
Lynch MJ, Raphael SS, Mellor LD, Spare PD, Hills P, Inwood MJH: Medical Laboratory Technology, Saunders Philadelphia, 1964, p 147
Zahler WL: Analytical polyacrylamide gel electrophoresis and molecular weight determination. In: Fleischer S and Packer L (eds), Methods in Enzymol. Academic Press, New York, 1974, vol 32 part B, pp 70–81
Burden EM, Yates CM, Reading HW: Investigation into structural integrity of lysosomes in the normal and dystrophic rat retina. Exp Eye Res 12:159–165, 1971
Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254, 1976
Adler AJ, Martin KJ: Lysosomal enzymes in the interphotoreceptor matrix: acid protease. Curr Eye Res 2:359–366, 1982/1983
Livrea MA, Tesoriere L, Bongiorno A, Valenza M: Occurence of retinaldehyde in the interphotoreceptor matrix in light and dark adapted bovine eye. Med Sci Res 15:107–108, 1987
Bridges CD, Alvarez RA: Selective loss of 11-cis vitamin A in an eye with hereditary chororioretinal degeneration similar to sector retinitis pigmentosa. Retina 2:256–260, 1982
Adler AJ, Martin KJ: Retinol-binding proteins in bovine interphotoreceptor matrix. Biochem Biophys Res Commun 108:1601–1608, 1982
Liou GI, Bridges CDB, Fong SL, Alvarez RA, Gonzales-Fernandez F: Vitamin A transport between retina and pigment epithelium-an interstitial protein carrying endogenous retinol (interstitial retinol-binding protein). Vision Res 22:1457–1467, 1982
Heller J: Intracellular retinol-binding proteins from bovine pigment epithelial and photoreceptor cell fractions. J Biol Chem 251:2952–2957, 1976
Bairati A, Orzalesi N: The ultrastructure of pigment epithelium and of the photoreceptor-pigment epithelium junction in the human retina. J Ultrastruc Res 9:484–496, 1963
Young RW. Shedding of discs from rod outer segments in the rhesus monkey. J Ultrastruc Res 34:190–203, 1971
Regan CM, De Grip WJ, Daemen FJM, Bonting SL: Degradation of rhodopsin by a lysosomal fraction of retinal pigment epithelium: biochemical aspects of the visual process. XLI. Exp Eye Res 30:183–191, 1980
Van Breugel PJGM, Daemen FJM, Bonting SL: Biochemical aspects of the visual process. XXIX. Effect of pronase on rod outer segment membranes and rhodopsin. Exp Eye Res 21:315–324, 1975
Borggreven JMPM, Daemen FJM, Bonting SL: Biochemical aspects of the visual process. VI. The lipid composition of native and hesane-extracted cattle rod outer segments. Biochim Biophys Acta 202:374–381, 1970
Miljanich GP, Dratz EA: Fatty acid composition and pairing in phospholipids of rod outer segments. In: Packer, L (ed) Methods in Enzymol. Academic Press, New York, 1982, vol 81, pp 806–815
Anderson RE, Maude MB, Zimmerman W: Lipids of ocular tissues. X. Lipids composition of subcellular fractions of bovine retina. Vision Res 15:1087–1090, 1975
Anderson RE, Lissandrello PM, Maude MB, Matthes MT: Lipids of bovine retinal pigment epithelium. Exp Eye Res 23:149–157, 1976
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Livrea, M.A., Bongiorno, A., Bono, A. et al. Analysis of a soluble lipid-protein complex carrying endogenous 11-cis retinaldehyde from bovine retinal pigment epithelium. Mol Cell Biochem 85, 181–189 (1989). https://doi.org/10.1007/BF00577113
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00577113