Abstract
A UV-sensitive mutant line of CHO cells, UV20, was shown to be phenotypically corrected to resistance by fusion with human lymphocytes or fibroblasts. Only human chromosome 19 correlated with the DNA repair phenotype of resistant hybrid clones and their resistant or sensitive subclones. This study demonstrates the mapping of a human repair gene by direct selection of complementing hybrids in the presence of a DNA-damaging agent (mitomycin C).
Similar content being viewed by others
Literature cited
Cleaver, J.E. (1983). InThe Metabolic Basis of Inherited Disease, 5th ed., (eds.) Stanbury, J.B., Wyngaarden, J.B., Fredrickson, D.S., Goldstein, J.L., and Brown, M.S. (McGraw-Hill, New York), pp. 1227–1248.
Busch, D.B., Cleaver, J.E., and Glaser, D.A. (1980).Somat. Cell Genet.6:407–418.
Thompson, L.H., Rubin, J.S., Cleaver, J.E., Whitmore, G.F., and Brookman, K. (1980).Somat. Cell Genet.6:391–405.
Thompson, L.H., Busch, D.B., Brookman, K., Mooney, C.L., and Glaser, D.A. (1981).Proc. Natl. Acad. Sci. U.S.A. 78:3734–3737.
Shiomi, T., Hieda-Shiomi, N., and Sato, K. (1982).Somat. Cell Genet. 8:329–345.
Thompson, L.H., Brookman, K.W., Dillehay, L.E., Mooney, C.L., and Carrano, A.V. (1982).Somat. Cell Genet. 8:759–773.
Hori, T., Shiomi, T., and Sato, K. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:5655–5659.
Rubin, J.S., Joyner, A.L., Bernstein, A., and Whitmore, G.F. (1983).Nature 306:206–208.
Brahe, C., and Serra, A. (1981).Somat. Cell Genet. 7:109–115.
Hashem, N., Bootsma, D., Keijzer, W., Greene, A., Coriell, L., Thomas, G. and Cleaver, J.E. (1980).Cancer Res. 40:13–18.
Harris, H., and Hopkinson, D.A. (1976).Handbook of Enzyme Electrophoresis in Human Genetics (North-Holland, Oxford).
Siciliano, M.J., and Shaw, C.R. (1976). InChromatographic and Electrophoretic Techniques, Vol. II (ed.) Smith, D. (Heinemann Medical Books Ltd., London), pp. 185–209.
Koch, G.A., Eddy, R.L., Haley, L.L., Byers, M.G., McAvoy, M., and Shows, T.B. (1983).Cytogenet. Cell Genet. 35:67–69.
Barker, R.F., and Hopkinson, D.A. (1978).Ann. Hum. Genet. London 42:143–151.
Shows, T.B., and 24 coauthors (1979).Cytogenet. Cell Genet. 25:96–116.
Burgerhout, W. (1975).Humangenetik 29:229–231.
Sahar, E., and Latt, S.A. (1980).Chromosoma (Berlin) 79:1–28.
Westerveld, A., Hoeijmakers, J.H.J., van Duin, M., de Wit, J., Odijk, H., Pastink, A., Wood, R.D., and Bootsma, D. (1984).Nature 310:425–429.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Thompson, L.H., Mooney, C.L., Burkhart-Schultz, K. et al. Correction of a nucleotide-excision-repair mutation by human chromosome 19 in hamster-human hybrid cells. Somat Cell Mol Genet 11, 87–92 (1985). https://doi.org/10.1007/BF01534738
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01534738