Skip to main content
SpringerLink
Log in

Cloning of DNA libraries from mouse Y chromosomes purified by flow cytometry

  • Published:
Somatic Cell and Molecular Genetics

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

To purify mouse Y chromosomes by flow cytometry, a male cell line containing the Robertsonian translocation Rb(9.19)163H has been established by SV40 transformation. Flow karyotypes obtained from these cells exhibit a well-isolated peak of fluorescence corresponding to the single Y chromosome, clearly distinct from that of chromosome 19. From this peak, 650,000 chromosomes were sorted, and two restriction fragment libraries were constructed from the DNA of the sorted chromosomes. The characterization of several Y-specific fragments has shown that the Y DNA was enriched at least 36-fold. Furthermore, given that there are likely homologies between the X and Y chromosomes, we can assume that this calculated value of the purification factor is an underestimation and that the Y DNA was more highly purified by flow sorting.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  1. De La Chapelle, A., Tippett, P.A., Wetterstrand, G., and Page, D. (1984).Nature 307:170–171.

    PubMed  Google Scholar 

  2. Guellaen, G., Casanova, M., Bishop, C., Geldwerth, D., Andre, G., Fellous, M., and Weissenbach, J. (1984).Nature 307:172–173.

    PubMed  Google Scholar 

  3. Page, D.C., De La Chapelle, A., and Weissenbach, J. (1985).Nature 315:224–226.

    PubMed  Google Scholar 

  4. Evans, E.P., Burtenshaw, M.D., and Cattanach, B.M. (1982).Nature 300:443–445.

    PubMed  Google Scholar 

  5. Singh, L., and Jones, K.W. (1982).Cell 28:205–216.

    PubMed  Google Scholar 

  6. Davis, R.M. (1981).J. Med. Genet. 18:161–195.

    PubMed  Google Scholar 

  7. Goodfellow, P., Banting, G., Sheer, D., Ropers, H.H., Caine, E., Ferguson-Smith, M.A., Povey, S., and Voss, R. (1983).Nature 302:346–349.

    PubMed  Google Scholar 

  8. Bishop, C., Guellaen, G., Geldwerth, D., Voss, R., Fellous, M., and Weissenbach, J. (1983).Nature 303:831–832.

    PubMed  Google Scholar 

  9. Lamar, E.E., and Palmer, D. (1984).Cell 37:171–177.

    PubMed  Google Scholar 

  10. Davies, K.E., Young, B.D., Elles, R.G., Hill, M.E., and Williamson, R. (1981).Nature 293:374–376.

    PubMed  Google Scholar 

  11. Krumlauf, R., Jeanpierre, M., and Young, B.D. (1982).Proc. Natl. Acad. Sci. U.S.A. 79:2971–2975.

    PubMed  Google Scholar 

  12. Kunkel, L.M., Tantravahi, V., Eisenhard, M., and Latt, S.A. (1982).Nucleic Acids Res. 10:1557–1578.

    PubMed  Google Scholar 

  13. Disteche, C.M., Kunkel, L.M., Lojewski, A., Orkin, S.M., Eisenhard, M., Sahar, B., Travis, B., and Latt, S.A. (1982).Cytometry 2:282–286.

    PubMed  Google Scholar 

  14. Baron, B., Métézeau, P., Bernheim, A., Berger, R., Guenet, J.-L., and Goldberg, M.E. (1984).Exp. Cell Res. 152:220–230.

    PubMed  Google Scholar 

  15. Disteche, C.M., Carrano, A.V., Ashworth, L.K., Burkhart-Shultz, K., and Latt, S.A. (1981).Cytogenet. Cell Genet. 29:189–197.

    PubMed  Google Scholar 

  16. Evans, E.P., Lyon, M.F., and Daglish, M. (1967).Cytogenetics 6:105–119.

    PubMed  Google Scholar 

  17. Sillar, R., and Young, B.D. (1981).J. Histochem. Cytochem. 29:74–78.

    PubMed  Google Scholar 

  18. Blumenthal, A.B., Dieden, J.D., Kapp, L.N., and Sedat, J.-N. (1979).J. Cell. Biol. 81:255–259.

    PubMed  Google Scholar 

  19. Daiger, S.P., Wildin, R.S. and Su, T.S. (1982).Nature 298:682–684.

    PubMed  Google Scholar 

  20. Murray, M. (1983). Phage lambda molecular cloning. Lambda II, (Cold Spring Harbor Laboratory. Cold Spring Harbor, New York), p. 295.

    Google Scholar 

  21. Rigby, P.W., Dieckmann, J.M., Rhodes, C., and Berg, P. (1977).J. Mol. Biol. 113:237–251.

    PubMed  Google Scholar 

  22. Southern, E.M. (1975).J. Mol. Biol. 98:503–517.

    PubMed  Google Scholar 

  23. Buys, C.H.C.M., Koerts, T., and Aten, J.A. (1982).Hum. Genet. 61:157–159.

    PubMed  Google Scholar 

  24. Griffith, J.K., Cram, L.S., Crawford, B.D., Jackson, P.J., Schilling, J., Schimke, T., Walters, R.A., Wilder, M.E., and Jett, J.H. (1984).Nucleic Acids Res. 12:4019–4034.

    PubMed  Google Scholar 

  25. Bishop, C., Guellaen, G., Geldwerth, D., Fellous, M., and Weissenbach, J. (1984).J. Mol. Biol. 173:403–417.

    PubMed  Google Scholar 

  26. Burgoyne, P.S. (1982).Hum. Genet. 61:85–90.

    PubMed  Google Scholar 

  27. Geldwerth, D., Bishop, C., Guellaen, G., Koenig, M., Vergnaud, G., Mandel, J.-L., and Weissenbach, J. (1985).Embo J. 4:1739–1743.

    PubMed  Google Scholar 

  28. Polani, P.E. (1982).Hum. Genet. 60:207–211.

    PubMed  Google Scholar 

  29. Simmler, M.-C., Rouyer, R., Vergnaud, G., Nystrom-lahti, M., Ngo, K.Y., De La Chapelle, A., and Weissenbach, J. (1985).Nature 317:692–697.

    PubMed  Google Scholar 

  30. Bishop, C., Boursot, R., Baron, B., Bonhomme, F., and Hatat, D. (1985).Nature 315:70–72.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Service Commun INSERM-PASTEUR “Trieur de Cellules”, France

    Bruno Baron, Philippe Métézeau & Michel E. Goldberg

  2. Institut Pasteur, Unité d'Immunogén'etique Humaine (Inserm U.276), 28 rue du Docteur Roux, 75724, Paris, Cedex 15, France

    Didier Hatat, Christa Roberts & Colin Bishop

Authors
  1. Bruno Baron
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Philippe Métézeau
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Didier Hatat
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Christa Roberts
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Michel E. Goldberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Colin Bishop
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Baron, B., Métézeau, P., Hatat, D. et al. Cloning of DNA libraries from mouse Y chromosomes purified by flow cytometry. Somat Cell Mol Genet 12, 289–295 (1986). https://doi.org/10.1007/BF01570788

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01570788

Keywords

Search

Navigation