Abstract
The genes encoding intermediate filament (IF) proteins are expressed in a cell-lineage restricted fashion. To analyze the regulation of such genes, we studied cytokeratin and vimentin expression in hepatoma × fibroblast hybrids. These hybrids continued to express both hepatoma cell-derived cytokeratins and fibroblast-specific vimentin. Furthermore, the cytokeratin subunits that were produced were exclusively of rat hepatoma origin. Thus, IF protein genes were neither extinguished nor activated in cell hybrids, providing evidence for regulation in cis.This behavior contrasts sharply with that of most tissue-specific genes, which tend to be regulated in transin hybrid cells.
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Gourdeau, H., Fournier, R.E.K. Cytokeratin gene expression in hepatoma hybrid cells: Evidence for regulation incis . Somat Cell Mol Genet 15, 359–366 (1989). https://doi.org/10.1007/BF01534974
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DOI: https://doi.org/10.1007/BF01534974