Abstract
Huntington's disease (HD) is tightly linked to genetic markers in 4p16.3. We have used a regional somatic cell hybrid mapping panel to isolate and map 25 cosmids to the proximal portion of 4p16.3 and 17 cosmids to the distal portion. The latter were positioned by long-range restriction mapping relative to previously mapped markers. One cosmid, L6 (D4S166), spans the critical breakpoint in the mapping panel that distinguishes proximal and distal 4p16.3. Four of the cosmids mapped distal toD4S90, the previous terminal marker on 4p, and stretched to within 75 kb of the telomere. Several of the cosmids that mapped between L6 andD4S90 were clustered near a number of previously isolated clones in a region with many NotI sites. Cosmid E4 (D4S168) was localized immediately proximal to the one remaining gap in the long-range restriction map of distal 4p16.3. Although pulsed field gel mapping with E4 failed to link the two segments of the map, the intervening gap was excluded as a potential site for theHD gene by genetic analysis.
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Literature cited
Martin, J.B., and Gusella, J.F. (1986).N. Engl. J. Med. 3151267–1276.
Gusella, J.F., Wexler, N.S., Conneally, P.M., Naylor, S.L., Anderson, M.A., Tanzi, R.E., Watkins, P.C., Ottina, K., Wallace, M.R., Sakaguchi, A.Y., Young, A.B., Shoulson, I., Bonilla, E., and Martin, J.B. (1983).Nature 306234–238.
Gusella, J.F., Tanzi, R.E., Anderson, M.A., Hobbs, W., Gibbons, K., Raschtchian, R., Gilliam, T.C., Wallace, M.R., Wexler, N.S., and Conneally, P.M. (1984).Science 2251320–1326.
Gilliam, T.C., Tanzi, R.E., Haines, J.L., Bonner, T.I., Faryniarz, A.G., Hobbs, W.J., MacDonald, M.E., Cheng, S.V., Folstein, S.E., Conneally, P.M., Wexler, N.S., and Gusella, J.F. (1987).Cell 50565–571.
MacDonald, M.E., Anderson, M.A., Gilliam, T.C., Tranebjaerg, L., Carpenter, N., Magenis, E., Hayden, M.R., Healey, S.T., Bonner, T.I., and Gusella, J.F. (1987).Genomics 129–34.
Smith, B., Skarecky, D., Bengtsson, U., Magenis, R.E., Carpenter, N., and Wasmuth, J.J. (1988).Am. J. Hum. Genet. 42335–344.
Robbins, C., Theilman, J., Youngman, S., Haines, J., Altherr, M.J., Harper, P.S., Payne, C., Junker, A., Wasmuth, J., and Hayden, M.R. (1989).Am. J. Hum. Genet. 44422–425.
MacDonald, M.E., Haines, J.L., Zimmer, M., Cheng, S.V., Youngman, S., Whaley, W.L., and Wexler, N.S. (1989).Neuron 3183–190.
Bucan, M., Zimmer, M., Whaley, W.L., Poustka, A., Youngman, S., Allitto, B.A., Ormondroyd, E., Smith, B., Pohl, T.M., MacDonald, M., Bates, G., Richards, J., Volinia, S., Gilliam, T.C., Sedlacek, Z., Collins, F.S., Wasmuth, J.J., Shaw, D.J., Gusella, J.F., Frischauf, A.M., and Lehrach, H. (1990).Genomics 61–15.
Wasmuth, J.J., Carlock, L.R., Smith, B., and Immken, L. (1986).Am. J. Hum. Genet. 39397–403.
Poustka, A., Rackwitz, H., Frischauf, A., and Hohn, B. (1984).Proc. Natl. Acad. Sci. U.S.A. 814129–4133.
Willard, H.F. (1985).Am. J. Hum. Genet. 37524–532.
Mulligan, R.C., and Berg, P. (1980).Science 2091422–1427.
Sandri-Goldin, R.M., Goldin, A.L., Levine, M., and Glorioso, J. (1983).Methods Enzymol. 101402–411.
Oates, D.C., and Patterson, D. (1977).Somat. Cell Genet. 3561–577.
Stanley, W., and Chu, E.H. (1978).Cytogenet. Cell Genet. 22228–231.
Youngman, S., Sarfarazi, M., Bucan, M., MacDonald, M.E., Smith, B., Zimmer, M., Gilliam, C., Frischauf, A.M., Wasmuth, J.J., Gusella, J.F., Lehrach, H., Harper, P., and Shaw, D. (1989).Genomics 5802–809.
Evans, G., and Wahl, G. (1987).Methods Enzymol. 152604–610.
Whaley, W.L., Michiels, F., MacDonald, M.E., Romano, D., Zimmer, M., Smith, B., Leavitt, J., Bucan, M., Haines, J.L., Gilliam, T.C., Zehetner, G., Smith, C., Cantor, C.R., Frischauf, A.M., Wasmuth, J.J., Lehrach, H., and Gusella, J.F. (1988).Nucleic Acids Res. 1611769–11780.
Gusella, J.F., Keys, C., Varsanyi-Breiner, A., Kao, F.T., Jones, C., Puck, T.T., and Housman, D. (1980).Proc. Natl. Acad. Sci. U.S.A. 772829–2833.
Sealey, P.G., Whittaker, P.A., and Southern, E.M. (1985).Nucleic Acids Res. 131905–1922.
Gilliam, T.C., Bucan, M., MacDonald, M.E., Zimmer, M., Haines, J.L., Cheng, S.V., Pohl, T.M., Meyers, R.H., Whaley, W.L., Allitto, B.A., Faryniarz, A., Wasmuth, J.J., Frischauf, A.M., Conneally, P.M., Lehrach, H., and Gusella, J.F. (1987).Science 238950–952.
Pohl, T., Zimmer, M., Macdonald, M.E., Smith, B., Bucan, M., Poustka, A., Volinia, S., Searle, S., Zehetner, G., Wasmuth, J.J., Gusella, J.F., Lehrach, H., and Frischauf, A. (1988).Nucleic Acids Res. 169185–9198.
Bates, G.P., MacDonald, M.E., Baxendale, S., Sedlacek, Z., Youngman, S., Romano, D., Whaley, W.L., Allitto, B.A., Poustka, A., Gusella, J.F., and Lehrach, H. (1990).Am. J. Hum. Genet. 46762–775.
Snell, R.G., Lazarou, L., Youngman, S., Quarrell, O.W.J., Wasmuth, J.J., Shaw, D.J., and Harper, P.S. (1989).J. Med. Genet. 26673–675.
Theilman, J., Kanani, S., Shiang, R., Robbins, C., Quarrell, O., Huggins, M., Hedrick, A., Weber, B., Collins, C., Wasmuth, J.J., Buetow, K., Murray, J.C., and Hayden, M.R. (1989).J. Med. Genet. 26676–681.
Gusella, J.F. (1986).Annu. Rev. Biochem. 55831–854.
Gusella, J.F., Tanzi, R.E., Bader, P.I., Phelan, M.C., Stevenson, R., Hayden, M.R., Hofman, K.J., Faryniarz, A.G., and Gibbons, K. (1985).Nature 31875–78.
Zabel, B.U., Naylor, S.L., Sakaguchi, A.Y., and Gusella, J.F. (1986).Cytogenet. Cell Genet. 42187–191.
Magenis, R.E., Gusella, J., Weliky, K., Olson, S., Haight, G., Toth-Fejel, S., and Sheehy, R. (1986).Am. J. Hum. Genet. 39383–392.
Wang, H.S., Greenberg, C.R., Hewitt, J., Kalousek, D., and Hayden, M.R. (1986).Am. J. Hum. Genet. 39392–396.
Landegent, J.E., Jansen in de Wal, N., Fisser-Groen, Y.M., Bakker, E., Van der Ploeg, M., and Pearson, P.L. (1986).Hum. Genet. 73354–357.
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Whaley, W.L., Bates, G.P., Novelletto, A. et al. Mapping of cosmid clones in Huntington's disease region of chromosome 4. Somat Cell Mol Genet 17, 83–91 (1991). https://doi.org/10.1007/BF01233207
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DOI: https://doi.org/10.1007/BF01233207