Zusammenfassung
Durch Sensibilisierung von Kaninchen mit pepsinverdautem menschlichen Gammaglobulin (Gammavenin®) wurde ein Serum gewonnen, das relativ kräftige Präcipitine gegen die Receptoren der H- und der L- (Lambda- und Kappa-)Polypeptidketten des Immunglobulin IgG enthält. In der immunelektrophoretischen Analyse kommen bei Verwendung dieses Antiserums im IgG-Bereich drei Linien zur Darstellung, die den präzipitierten H- und L-Ketten des komplettenIg G-Moleküls entsprechen. Es wird demonstriert, daß die nach der beschriebenen Methode gewonnenen Antiseren für die immunelektrophoretische Analyse von Paraproteinen erhebliche Vorteile besitzen.
Summary
A serum was obtained by sensibilisation of rabbits with pepsin digested human gamma-globulin (Gammavenin®), which contained relatively strong precipitants against the receptors of the h-chain and l-chains (kappa and lambda) of the immunoglobulin IgG. In the immunoelectrophoretic analysis three lines in the IgG-area can thus be demonstrated, which correspond to the h- and l-polypeptid-chains of the complete IgG-molecule. It has been shown, that those antisera are dvantageous for the immunoelectrophoretic analysis of paraproteins.
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Mit Unterstützung der Deutschen Forschungsgemeinschaft.
Mit technischer Assistenz vonI. Lichtenstein undI. Fritz.
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Bläker, F., Fischer, K. Präparation eines Antiserums gegen enzymatisch gespaltenes Gammaglobulin (Gammavenin®). Klin Wochenschr 45, 494–495 (1967). https://doi.org/10.1007/BF01717439
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DOI: https://doi.org/10.1007/BF01717439