Skip to main content
SpringerLink
Log in

Untersuchungen zum Wirkungsmechanismus von heterologen Antilymphocyten-Globulinen

  • Kurze Wissenschaftliche Mitteilungen
  • Published:
Klinische Wochenschrift Aims and scope Submit manuscript

Zusammenfassung

Anti-Maus-Thymus-Globulin (AMT-IgG) vom Kaninchen, präparativ durch fraktionierte Fällung mit (NH4)2SO4 und Diaminoacridin hergestellt, unterdrückt die Abstoßung von zweiten allogeneischen Hauttransplantaten (C57/B1/6 auf A/NMRI) in sensibilisierten Mäusen vollständig. Die Verdauung des aktiven IgG-Globulins mit Pepsin ergibt ein bivalentes agglutinierendes Antikörperfragment (F (ab′(2), das keine immunsuppressive Aktivität mehr in vivo besitzt. Die Überlebenszeit primärer Hauttransplantate von C57/B1/6 auf A/NMRI wird durch Injektionen vonF (ab′) 2-Fragmenten des AMT-IgG nicht verlängert. Die mitF (ab′)2 behandelten Mäuse zeigen keine Lymphopenie. Es wird daraus geschlossen, daß ein integresF c -Fragment für die immunsuppressive Wirkung der heterologen Antilymphocyten-Globuline notwendig ist. Die Frage, ob der spezifische Reaktionsmechanismus komplementabhängig ist oder durch die cytophile Aktivität desF c -Fragments bewirkt wird, bleibt offen.

Summary

Anti-mouse-thymus globulin (AMT-IgG) prepared from rabbit serum by fractional precipitation with (NH4)2SO4 and diaminoacridin abrogates completely second set reactions against allogeneic skin grafts when injected into A/NMRI mice. Pepsin-digestion of this active globulin yields bivalent antibody fragments (F (ab′)2), which still agglutinate thymocytes in vitro but are no longer effective in prolonging survival of primary allogeneic skin grafts. It is concluded that the immunosuppressive activity of the herologous antilymphocytic globulin is conveyed by theF c fragment. The specific mechanism by which AMT-IgG acts may be complement-dependent, e.g. cytotoxic, or may be induced by cytophilic properties of the antibodies. Injections ofF (ab′) 2 fragments do not produce peripheral lymphopenia.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literatur

  1. Anderson, N. F., andK. James: Effect of anti-rat lymphocyte antibody on humoral antibody formation. Nature (Lond.)213, 1195 (1967).

    Google Scholar 

  2. Berken, A., Benacerraf: Properties of antibodies cytophilic for macrophages. J. exp. Med.123, 119 (1966).

    Google Scholar 

  3. Brambell, F. W. R., W. A. Hemmings, C. L. Oakley, andR. R. Porter: The relative transmission of the fractions of papain hydrolyzed homologous γ-globulins from the uterine cavity to the foetal circulation in the rabbit. Proc. roy. Soc. B151, 478 (1960).

    Google Scholar 

  4. Heide, H., u.K. Haupt: Behringwerke Mitt.43, 161 (1964). Zit.H. E. Schultze, andJ. F. Heremans, Molecular biology of human proteins. Amsterdam 1966.

    Google Scholar 

  5. Levey, R. H., andP. B. Medawar: Nature and mode of action of antilymphocytic antiserum. Proc. nat. Acad. Sci. (Wash.)56, 1130 (1967).

    Google Scholar 

  6. Monaco, A. P., M. L. Wood, J. G. Gray, andP. S. Russel: Studies on heterologous anti-lymphocyte serum in mice. II. Effect on the immune response. J. Immunol.96, 229 (1966).

    Google Scholar 

  7. Nagaya, H., andH. O. Sieker: Allograft survival: Effect of antiserums to thymus glands and lymphocytes. Science150, 1181 (1965).

    Google Scholar 

  8. Nisonoff, A.: Enzymatic digestion of rabbitγ-globulin and antibody and chromatography of digestion products. In: Methods in medical research, vol. 10, p. 134. Chicago 1964.

  9. Ovary, Z., andF. J. Karush: Sensitizing and combining capacity in vivo of fractions separated from papain digests of antihapten antibody. J. Immunol.86, 146 (1961).

    Google Scholar 

  10. Pichlmayr, R.: Herstellung und Wirkung heterologer Antihundelymphozytenseren. Z. Ges. exp. Med.143, 161 (1967).

    Google Scholar 

  11. Riethmüller, G., P. Rieber u.D. Riethmüller: In Symposium über Transplantationsimmunologie, April 1967. Z. Immun.-Forsch. (1967) (im Druck).

  12. Riethmüller, G., P. Rieber, andD. Riethmüller: Effect of intact and of pepsin-digested anti-mouse thymus IgG on antibody-forming spleen cells and on the homograft reaction. Abstracts of the First Internat. Congr. of the Transplantation Congr., p. 155, Paris 1967.

  13. Taranta, A., andE. C. Franklin: Complement fixation by antibody fragments. Science134, 1981 (1961).

    Google Scholar 

  14. Turck, J. L., andM. L. Willoughby: Central and peripheral effects of antilymphocyte sera. Lancet1967 I, 251.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Medizinische Universitätsklinik Tübingen, Germany

    G. Riethmüller, P. Rieber & D. Riethmüller

Authors
  1. G. Riethmüller
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Rieber
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Riethmüller
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Riethmüller, G., Rieber, P. & Riethmüller, D. Untersuchungen zum Wirkungsmechanismus von heterologen Antilymphocyten-Globulinen. Klin Wochenschr 46, 44–46 (1968). https://doi.org/10.1007/BF01725300

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01725300

Search

Navigation