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Radio-Retentionselektrophorese: ein quantitatives und spezifisches Verfahren zur Bestimmung einzelner Proteine im Nanogrammbereich

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Zusammenfassung

Wie am Beispiel des Humanalbumins gezeigt wird, lassen sich durch Kombination von Elektrophorese in antikörperhaltigem Agar mit Autoradiographie (Radio-Retentionselektrophorese) wenige Nanogramm Antigen quantitativ und spezifisch nachweisen. Technik und Anwendungsbereich der Methodik sowie einige Resultate der Blutspiegelbestimmung werden mitgeteilt.

Summary

Electrophoresis in agar containing antibodies can be combined with subsequent autoradiography (radioretention electrophoresis) which allows quantitative and specific determination of a few nanograms of antigen, e.g. human albumin. Techniques and application range of the method as well as some results of blood level determinations are reported.

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Literatur

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Authors and Affiliations

  1. Pharmakologisches Institut der Justus Liebig-Universität Gießen, Germany

    E. Habermann, K. -O. Räker & D. Arndts

Authors
  1. E. Habermann
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  2. K. -O. Räker
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  3. D. Arndts
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Habermann, E., Räker, K.O. & Arndts, D. Radio-Retentionselektrophorese: ein quantitatives und spezifisches Verfahren zur Bestimmung einzelner Proteine im Nanogrammbereich. Klin Wochenschr 46, 46–47 (1968). https://doi.org/10.1007/BF01725301

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  • DOI: https://doi.org/10.1007/BF01725301

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