Zusammenfassung
Die Cytotoxizität von Antihumanlymphocytenglobulin auf menschliche Monocyten aus dem peripheren Blut wurde untersucht. Es zeigte sich, daß alle untersuchten Seren gegen Monocyten bei Gegenwart von Komplement cytotoxisch waren, allerdings in viel geringerem Maße als gegenüber Lymphocyten. Der cytotoxische Effekt gegenüber Monocyten konnte durch Absorption mit Lymphocyten entfernt werden.
Bei Zusatz von ALG sensibilisierten Lymphocyten zu Monocyten kam es zur Rosettenbildung, die in Gegenwart von Komplement bis zu hohen ALG-Verdünnungen nachweisbar war.
Summary
The cytotoxicity of antihumanlymphocytic globulin against human monocytes isolated from peripheral blood was investigated. In the presence of complement the ALG preparations tested exhibited a cytotoxic effect against monocytes, which however was much less pronounced than against lymphocytes. By absorbing the sera with lymphocytes the cytotoxic effect against monocytes was also removed. If lymphocytes sensitized with ALG were added to monocytes rosette formation became demonstrable and in the presence of complement was detected up to high ALG dilutions.
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Michlmayr, G., Huber, H. Zur Wirkung von Antihumanlymphocytenglobulin auf menschliche Monocyten in vitro. Klin Wochenschr 47, 1283–1285 (1969). https://doi.org/10.1007/BF01487559
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DOI: https://doi.org/10.1007/BF01487559