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Gelfiltration von Serumlipoproteinen mit Agarose

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Zusammenfassung

Sepharose Typ 2 B, ein käufliches Gel aus 2%igen Agaroseperlen, wurde zur gelchromatographischen Trennung hyperlipämischer Seren und isolierter Lipoproteinfraktionen verwendet. Die Lipoproteine der FlotationsklassenS f >400 werden von dem Gel ausgeschlossen. Von den Lipoproteinen der KlassenS f 20–400 undS f 0–20 gewinnt man breite, einander überschneidende Größenverteilungsmuster mit definierten K d -Werten. Bei den verschiedenen Typen der Hyperlipoproteinämien zeigen die Elutionskurven der Gesamtseren diagnostisch verwertbare Unterschiede. Durch Agarose-Gelfiltration lassen sich Proteinverunreinigungen aus Rohfraktionen von Präbeta- und Betalipoproteinen auf schonende Weise entfernen.

Summary

Hyperlipaemic sera and lipoprotein fractions purified by preparative ultracentrifugation were examined by gel chromatography on Sepharose 2 B (2 per cent agarose). Lipoproteins with flotation constantsS f >400 are excluded by the gel. Lipoprotein classes ofS f 20–400 andS f 0–20 show broad and mutually overlapping elution peaks with well definedK d values. Sera of several types of hyperlipoproteinaemia show different elution curves which may be used for diagnostic purposes. Protein contaminations of lipoprotein fractions purified by ultracentrifugation can be separated by agarose 2 B gel filtration.

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Authors and Affiliations

  1. Medizinische Universitätsklinik (Ludolf Krehl-Klinik) Heidelberg, Germany

    M. Hollmann, J. Papenberg & W. Piper

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  1. M. Hollmann
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  2. J. Papenberg
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  3. W. Piper
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Hollmann, M., Papenberg, J. & Piper, W. Gelfiltration von Serumlipoproteinen mit Agarose. Klin Wochenschr 48, 493–497 (1970). https://doi.org/10.1007/BF01485102

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  • DOI: https://doi.org/10.1007/BF01485102

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