Skip to main content
SpringerLink
Log in

Enzymaktivitäten im Fettgewebe bei Hyperlipidämie

  • Kurze Wissenschaftliche Mitteilungen
  • Published:
Klinische Wochenschrift Aims and scope Submit manuscript

Zusammenfassung

Im subcutanen Fettgewebe von 27 Patienten mit Hyperlipidämien verschiedener Genese wurden die Aktivitäten von 8 Schlüsselenzymen bestimmt (Glucose-6-Phosphat-Dehydrogenase, 6-Phosphogluconat-Dehydrogenase, Hexokinase, Phosphofructokinase, Pyruvatkinase, Glycerin-3-Phosphat-Dehydrogenase, Malatdehydrogenase, Glucophosphomutase). Dabei fand sich eine hochsignifikante Herabsetzung der Glucose-6-Phosphat-Dehydrogenase- und der Hexokinase-Aktivität, woraus auf eine verminderte Glucoseverwertung geschlossen werden kann. In Verbindung damit spricht die deutlich erniedrigte Aktivität der Glycerin-3-Phosphat-Dehydrogenase für eine gleichzeitig herabgesetzte Lipogenese im menschlichen Fettgewebe.

Summary

The activities of 8 key-enzymes (glucose-6-phosphate-dehydrogenase, 6-phosphogluconate-dehydrogenase, hexokinase, phosphofructokinase, pyruvate kinase, glycerol-3-phosphate-dehydrogenase, malate dehydrogenase, phosphoglucomutase) have been estimated in the subcutaneous adipose tissue of 27 patients with hyperlipemia of different origin. We found that the activities of glucose-6-phosphate-dehydrogenase and hexokinase were diminished significantly, what means a lowered utilisation of glucose. From these results in connection with a diminished activity of glycerol-3-phosphate-dehydrogenase it can be concluded that lipogenesis in human adipose tissue is diminished at the same time.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literatur

  1. Björntorp, P., Schersten, T., Gottfries, A.: Effects of glucose infusions on adipose tissue lipogenesis in man. Acta med. scand.183, 565 (1968).

    Google Scholar 

  2. Galton, D. J.: Lipogenesis in human adipose tissue. J. Lipid Res.9, 19 (1968).

    Google Scholar 

  3. —— Regulation of supply of glycerol phosphate for lipogenesis in human adipose tissue. Clin. Sci.36, 505 (1969).

    Google Scholar 

  4. Griglio, S., Goranov, I., Lavau, M., Lowy, R.: Étude des activités enzymatiques du foie et du tissue adipeux épididymaire du rat Wistar au cours de l'administration à long terme d'un régime hyperlipidique. Enzym. biol. clin.10, 187 (1969).

    Google Scholar 

  5. Kahlenberg, A., Kalant, N.: The effect of insulin on human tissue. Canad. J. Biochem.42, 1623 (1964).

    Google Scholar 

  6. Kuo, P. T., Feng, L., Cohen, N. N., Fitts, W. T., Miller, L. D.: Dietary carbohydrates in hyperlipemia (Hyperglyceridemia); hepatic and adipose tissue lipogenic activities. Amer. J. clin. Nutr.20, 116 (1967).

    Google Scholar 

  7. Leveille, G. A.: Influence of dietary fat and protein on metabolic and enzymatic activities in adipose tissue of meal-fed rats. J. Nutr.91, 25 (1967).

    Google Scholar 

  8. —— Influence of dietary fat level on the enzymatic and lipogenic adaptations in adipose tissue of meal-fed rats. J. Nutr.91, 267 (1967).

    Google Scholar 

  9. McLean, P., Brown, J., Greenbaum, A. L.: Hormonal control of carbohydrate metabolism of adipose tissue. In: Carbohydrate metabolism and its disorders (ed. F. Dickens, P. J. Randle, and W. J. Whelan), p. 397. London-New York: Acad. Press. 1968.

    Google Scholar 

  10. Rognstad, R., Katz, J.: The balance of pyridine nucleotides and ATP in adipose tissue. Proc. nat. Acad. Sci. (Wash.)55, 1148 (1966).

    Google Scholar 

  11. Rud, C.: The hexokinase activity in adipose tissue. An investigation of mice and men. Rep. Steno Hosp.9, 13 (1960).

    Google Scholar 

  12. Schwandt, P., Doerr, H. W., Krone, W., Werner, S.: Aktivitätsbestimmung von Schlüsselenzymen im menschlichen Fettgewebe. Klin. Wschr. (im Druck).

  13. Shrago, E., Glennon, J. A., Gordon, E. S.: Enzyme studies in human liver and adipose tissue. Nature (Lond.)212, 1263 (1966).

    Google Scholar 

  14. —— Spennetta, T., Gordon, E.: Fatty acid synthesis in human adipose tissue. J. biol. Chem.244, 2761 (1969).

    Google Scholar 

  15. Handel, E. van, Zilversmit, D. B.: Micromethod for direct determination of serum triglycerides. J. Lab. clin. Med.50, 152 (1957).

    Google Scholar 

  16. Zöllner, N., Kirsch, K.: Über die quantitative Bestimmung von Lipoiden (Mikromethode) mittels der vielen natürlichen Lipoiden (allen bekannten Plasmalipoiden) gemeinsamen Sulfophosphoranillin-Reaktion. Z. ges. exp. Med.13, 545 (1962).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. I. Medizinische Universitätsklinik München, Germany

    W. Krone, H. W. Doerr & P. Schwandt

Authors
  1. W. Krone
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. W. Doerr
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Schwandt
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Krone, W., Doerr, H.W. & Schwandt, P. Enzymaktivitäten im Fettgewebe bei Hyperlipidämie. Klin Wochenschr 48, 503–504 (1970). https://doi.org/10.1007/BF01485108

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01485108

Search

Navigation