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Trennung der intestinalen β-Galactosidasen beim lactose-intoleranten Erwachsenen durch Ultrazentrifugation im Dichtegradienten

Separation of intestinal β-galaclosidases by ultracentrifugation in a density gradient in biopsies from lactose-intolerant adults

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Summary

In 11 adult probands with lactose intolerance the intestinalβ-galactosidases were separated in a density gradient by ultracentrifugation. The “neutral” brush border lactase and the “acid” lysosomal lactase were identified in the biopsy material of all 11 subjects. In comparison with lactosetolerant adults the brush border lactase was markedly diminished. The hetero-β-galactosidase which is present as a slowly sedimenting enzyme in lactose-tolerant adults, was absent in all of the 11 subjects.

Zusammenfassung

Bei 11 erwachsenen Versuchspersonen mit Lactose-Intoleranz konnten bei der Trennung der intestinalenβ-Galactosidasen im Dichtegradienten die „neutrale“ Bürstensaum-Lactase und die „saure“ lysosomale Lactase nachgewiesen werden. Im Vergleich zu lactose-toleranten Erwachsenen war die Bürstensaum-Lactase stark vermindert. Die Hetero-β-Galactosidase, die bei lactose-toleranten Erwachsenen als langsam sedimentierendes Enzym nachweisbar ist, fehlte bei allen 11 Versuchspersonen.

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Authors and Affiliations

  1. Universitäts-Kinderklinik Bonn, Germany

    H. W. Rotthauwe, G. Flatz, D. Emons & A. Heisig

Authors
  1. H. W. Rotthauwe
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  2. G. Flatz
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  3. D. Emons
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  4. A. Heisig
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Additional information

Diese Arbeit wurde durch die Stiftung Volkswagenwerk und durch das Landesamt für Forschung Nordrhein-Westfalen unterstützt.

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Rotthauwe, H.W., Flatz, G., Emons, D. et al. Trennung der intestinalen β-Galactosidasen beim lactose-intoleranten Erwachsenen durch Ultrazentrifugation im Dichtegradienten. Klin Wochenschr 50, 260–261 (1972). https://doi.org/10.1007/BF01486533

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  • DOI: https://doi.org/10.1007/BF01486533

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