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Untersuchung der DNS-abhängigen RNS-Polymerase aus menschlichen Leukocyten in einem einfachen zellfreien System

Determination of the DNA-dependent RNA polymerase from human leukocytes in a simple cell-free system

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Summary

Lymphocytes and leukocytes (lymphocytes + granulocytes), isolated from 10–40 ml of heparinized venous blood, are homogenized, and centrifuged for 15 min at 1000 g. The resuspended pellet, consisting of nuclei and nuclear debris, exhibits ca. 70% of the DNA-depenent RNA polymerase activity of the homogenate. The reaction depends on added DNA template and the presence of all four ribonucleoside triphosphates. In chronic lymphatic leukaemia, chronic myelocytic leukemia, and Hodgkin's disease, the activity of the DNA-dependent RNA polymerase is higher than in normal controls.

Zusammenfassung

Lymphocyten und Leukocyten (Lymphocyten + Granulocyten) werden aus 10–40 ml heparinisiertem Venenblut isoliert, homogenisiert und 15 min bei 1000 g zentrifugiert. In dem aus Zellkernen und Kerntrümmern bestehenden Sediment lassen sich nach Resuspension ca. 70% der DNA-abhängigen RNS-Polymerase-Aktivität des Homogenats nachweisen. Die Reaktion ist von zugesetzter DNS und der Gegenwart aller vier Ribonucleosid-Triphosphate abhängig. Bei chronisch lymphatischer Leukämie, chronisch myeloischer Leukämie und Morbus Hodgkin findet sich unter diesen Bedingungen eine höhere spezifische Aktivität der DNS-abhängigen RNS-Polymerase als bei Normalpersonen.

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Authors and Affiliations

  1. II. Medizinische Universitätsklinik, I. Medizinische Universitätsklinik und Molekularbiologischhämatologische Arbeitsgruppe der Universitäts-Kinderklinik Hamburg-Eppendorf, Germany

    M. Garbrecht, R. Mertelsmann & G. Schöch

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  2. R. Mertelsmann
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  3. G. Schöch
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Garbrecht, M., Mertelsmann, R. & Schöch, G. Untersuchung der DNS-abhängigen RNS-Polymerase aus menschlichen Leukocyten in einem einfachen zellfreien System. Klin Wochenschr 51, 730–734 (1973). https://doi.org/10.1007/BF01468365

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