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Enzymcytochemische Untersuchung der Gelenkpunktatzellen

Cytochemical observation in synovial fluid cells

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Summary

Acid phosphatase, ß-glucuronidase, alpha-naphthylacetatesterase, peroxidase and PAS-reaction were investigated by cytochemical enzyme reactions in synovial fluid cells of 42 samples from knee joints with various rheumatic diseases. These were calculated by a semi-quantitative method. The activity index of acid phosphatase and ß-glucuronidase was increased in lymphocytes of inflammatory synovial fluids. This finding is discussed in view of a possible in vivo stimulation or transformation of the lymphocytes. Concerning the monocytes there was an increase of the activity index of alpha-naphthylacetatesterase and a decrease of ß-glucuronidase in arthritic joint diseases.

Zusammenfassung

An 42 Kniegelenkspunktaten verschiedenster rheumatischer Erkrankungen wurden enzymcytochemisch die saure Phosphatase, Beta-Glukuronidase, Alpha-Naphthylacetatesterase, Peroxydase und PAS-Reaktion in den Zellen der Synovia bestimmt und nach einem semiquantitativen Verfahren ausgewertet. Bei arthritischen Gelenkergüssen war der Aktivitätsindex der sauren Phosphatase und Beta-Glukuronidase in den Lymphozyten erhöht. Dieser Befund wird im Hinblick auf eine mögliche in vivo Stimulation bzw. Transformation der Lymphozyten diskutiert. In den Monozyten fiel bei Arthritiden eine Erhöhung des Aktivitätsindex der Alpha-Naphthylacetatesterase und Erniedrigung der Beta-Glukuronidase auf.

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Authors and Affiliations

  1. Abteilung für Krankheiten der Bewegungsorgane und des Stoffwechsels, Germany

    H. Zeidler, M. Hoek, J. Odriozola & B. Dommes

  2. Abteilung für Haematologie, Department Innere Medizin, Medizinische Hochschule Hannover, Germany

    H. Zeidler, M. Hoek, J. Odriozola & B. Dommes

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  1. H. Zeidler
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  2. M. Hoek
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  3. J. Odriozola
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  4. B. Dommes
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Zeidler, H., Hoek, M., Odriozola, J. et al. Enzymcytochemische Untersuchung der Gelenkpunktatzellen. Klin Wochenschr 52, 699–700 (1974). https://doi.org/10.1007/BF01468482

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  • DOI: https://doi.org/10.1007/BF01468482

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