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Immunologischer Nachweis von Creatinkinase-MB im Serum beim Myokardinfarkt

Determination of creatine phosphokinase-MB in the serum of patients with myocardial infarction by an immunological method

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Summary

The immunological method of determining creatine phosphokinase-MB in the serum of patients with myocardial infarction described here is based on the differential measurements of CK-activities before and after a specific immuno-precipitation of the CK-MB-type. The minimum activity of the CK-MB-type which it is possible to determine with this method is 4% of the total activity. In patients with clinically confirmed myocardial infarctions 1–15% (mean 7.8%) of the total activity can be calculated as CK-activity of the MB-type on the first/second day(s) after the infarction. In patients with increased total CK-activity and without verified infarction the CK-MB content does not differ significantly from zero. The differences between the two groups are statistically significant. In patients with myocardial reinfarction the CK-MB-activity is higher than that after the first infarction. The immunological method to determine creatine-phosphokinase isoenzyme MB is of differential-diagnostic value in myocardial infarction.

Zusammenfassung

Es wird über den Nachweis von Creatinkinase-MB im Serum beim Myokardinfarkt mit einer immunologischen Methode berichtet. Die Methode beruht auf der differentiellen Bestimmung der Creatinkinase-Aktivitäten vor und nach spezifischer Immunpräcipitation von Creatinkinase-MB. Die Nachweisgrenze der Creatinkinase-MB-Aktivität mit dieser Methode liegt bei 4% der Creatinkinase-Gesamtaktivität. Bei Patienten mit klinisch gesichertem Myokardinfarkt kann am 1./2. Tag nach dem Infarkt 1–15% (Mittel 7,8%) der Creatinkinase-Gesamtaktivität als CK-MB-Aktivität nachgewiesen werden. Bei Patienten mit erhöhter CK-Gesamtaktivität ohne gesicherten Infarkt ist der CK-MB-Anteil nicht signifikant von Null unterschieden. Die Unterschiede der beiden Gruppen sind statistisch signifikant. Bei Patienten mit Reinfarkt findet sich ein höherer Anteil von CK-MB-Aktivität als nach Erstinfarkt. Die immunologische Nachweismethode für Creatinkinase-MB hat differentialdiagnostische Bedeutung beim Myokardinfarkt.

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Literatur

  1. Jockers-Wretou, E., Pfleiderer, G.: Quantitation of creatine kinase isoenzymes in human tissues by an immunological method. Hoppe-Seylers Z. physiol. Chem. (1975), im Druck

  2. Smith, A.F.: Separation of tissue and serum creatine kinase isoenzymes on polyacrylamide gel slabs. Clin. chim. Acta39, 351 (1973)

    Google Scholar 

  3. Witteveen, S.A.G.J., Sobel, B.E., DeLuca, M.: Kinetic properties of the isoenzymes of human creatine phosphokinase. Proc. nat. Acad. Sci. (Wash.)71, 1384 (1974)

    PubMed  Google Scholar 

  4. Somer, H., Konttinen, A.: A method allowing the quantitation of serum creatine kinase isoenzymes. Clin. chim. Acta36, 531 (1972)

    PubMed  Google Scholar 

  5. Goto, I., Katsuki, S.: Creatine phosphokinase isoenzymes in pathological human serum. Clin. chim. Acta30, 795 (1970)

    PubMed  Google Scholar 

  6. Roe, C.R., Limbird, L.E., Wagner, G.S., Nerenberg, S.T.: Combined isoenzyme analysis in the diagnosis of myocardial injury: Application of electrophoretic methods for the detection and quantitation of the creatine phosphokinase MB isoenzyme. J. Lab. clin. Med.80, 577 (1972)

    PubMed  Google Scholar 

  7. Anido, V., Conn, R.B., Mengoli, H.F., Anido, G.: Diagnostic efficacy of myocardial creatine phosphokinase using polyacrylamide disk gel electrophoresis. Amer. J. clin. Path.61, 599 (1974)

    Google Scholar 

  8. Sherwin, A.L., Siber, G.R., Elhilali, M.M: Fluorescence technique to demonstrate creatine phosphokinase isoenzymes. Clin. chim. Acta17, 245 (1967)

    PubMed  Google Scholar 

  9. Madsen, A.: Creatine phosphokinase isoenzymes in human tissue with special reference to brain extract. Clin. chim. Acta36, 17 (1972)

    PubMed  Google Scholar 

  10. Roberts, R., Henry, P.D., Witteveen, S.A.G.J., Sobel, B.E.: Quantification of serum creatine phosphokinase isoenzyme activity. Amer. J. Cardiol.33, 650 (1974)

    PubMed  Google Scholar 

  11. Karlsson, C., Karlsson, J., Anderson, U.B.: Gel-elektrofocusing for the separation of isoenzymes in human skeletal muscle. Proc. Aust. biochem. Soc.7, 16 (1974)

    Google Scholar 

  12. Mercer, D.W.: Separation of tissue and serum creatine kinase isoenzymes by ion-exchange column chromatography. Clin. Chem.20, 36 (1974)

    PubMed  Google Scholar 

  13. Leunis, J.C.: Séparation chromatographique des isoenzymes de la créatine-phospho-kinase du serum normal et après infarctus du myocarde. Ann. Biol. clin.31, 369 (1973)

    Google Scholar 

  14. Leunis, J. C., Etienne, J., Brauman, J.: Aleas des mesures d'activite enzymatique dans le sérum en routine. Acta clin. belg.29, 71 (1974)

    PubMed  Google Scholar 

  15. Würzburg, U., Wilz, I., Hennrich, N., Lang, H.: Quantitative immunologische Bestimmung der Aldolase-Isoenzyme im Serum. Z. klin. Chem.12, 176 (1974)

    Google Scholar 

  16. Kentel, H.J., Okabe, K., Jacobs, H.K., Ziter, F., Maland, L., Kuby, S.A.: Studies on adenosine triphosphate transphosphorylases. XI. Isolation of the crystalline adenosine triphosphate-creatine transphosphorylases from the muscle and brain of man, calf, and rabbit; and a preparation of their enzymatically active hybrids. Arch. Biochem. Biophys.150, 648 (1972)

    PubMed  Google Scholar 

  17. Takahashi, K., Ushikubo, S., Oimomi, M., Shinko, T.: Creatine phosphokinase isozymes of human heart muscle and skeletal muscle. Clin. chim. Acta38, 285 (1972)

    PubMed  Google Scholar 

  18. Awdeh, Z.L., Williamson, A.R., Askonas, B.A.: Isoelectric focusing in polyacrylamide gel and its application to immunoglobulins. Nature (Lond.)219, 66 (1968)

    PubMed  Google Scholar 

  19. Herbert, W.J.: In: Handbook of experimental immunology (D.M. Weir, ed.), 2. Aufl., Kap. 20. Oxford: Blackwell Scientific Publications 1973

    Google Scholar 

  20. Deutsche Gesellschaft für Klinische Chemie: Empfehlungen der Deutschen Gesellschaft für Klinische Chemie. Standardisierung von Methoden zur Bestimmung von Enzymaktivitäten in biologischen Flüssigkeiten. Z. klin. Chem.8, 658 (1970);10, 182 (1972)

  21. Hunter, W.M.: In: Handbook of experimental immunology (D.M. Weir, ed.), 2. Aufl., Kap. 17. Oxford: Blackwell Scientific Publications 1973

    Google Scholar 

  22. Sidell, F.R., Culver, D.L., Kaminskis, A.: Serum creatine phosphokinase activity after intramuscular injection. J. Amer. med. Ass.229, 1894 (1974)

    Google Scholar 

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Authors and Affiliations

  1. Klinisch-chemisches Institut am Städtischen Krankenhaus München-Harlaching, Germany

    D. Neumeier & M. Knedel

  2. Biochemische Forschung, E. Merck, Postfach 41 19, 61 Darmstadt

    U. Würzburg, N. Hennrich &  H. Lang

Authors
  1. D. Neumeier
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  2. M. Knedel
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  3. U. Würzburg
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  4. N. Hennrich
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  5. H. Lang
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Neumeier, D., Knedel, M., Würzburg, U. et al. Immunologischer Nachweis von Creatinkinase-MB im Serum beim Myokardinfarkt. Klin Wochenschr 53, 329–333 (1975). https://doi.org/10.1007/BF01469060

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