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The human peripheral lymphocyte—a model system for studying the combined effect of psoralen plus black light

Der periphere menschliche Lymphocyt — ein Modellsystem für die Untersuchung der kombinierten Wirkung von Psoralen und langwelligem UV-Licht

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Zusammenfassung

Es wurde die Wirkung von Psoralen zusammen mit langwelligem ultraviolettem Licht (UVA) auf den3H-Thymidin-Einbau PHA-stimulierter humaner Lymphocyten untersucht. Die PHA-induzierte Lymphocytentransformation wurde durch Psoralen plus UVA gehemmt, abhängig sowohl von der Psoralenkonzentration als auch der Einwirkdauer der UVA-Bestrahlung. Die Hemmung der DNA-Synthese in den stimulierten Lymphocyten erfolgte bei Psoralenkonzentrationen wie sie auch im Serum von Patienten erwartet werden können, die sich einer systemischen Photochemotherapie unterziehen. Ohne UVA-Bestrahlung war bei diesen Psoralenkonzentrationen kein Effekt auf die DNA-Synthese nachweisbar. Ebenso erwies sich alleinige UVA-Bestrahlung ohne Psoralenzusatz als wirkungslos auf das Wachstum PHA-stimulierter Lymphocytenkulturen.

Summary

The effect of psoralen plus long wavelength ultraviolet light (UVA) on3H-thymidine uptake of PHA stimulated human lymphocytes was investigated. PHA induced lymphocyte transformation was inhibited by the combined action of psoralen and UVA irradiation in a dose related manner. Inhibition of DNA-synthesis occurred at concentrations of psoralen that can be expected in the serum of patients treated by systemic photochemotherapy. No effect was noted at these psoralen concentrations in the absence of UVA irradiation. Also did UVA irradiation in the absence of psoralen not inhibit3H-thymidine incorporation into PHA stimulated lymphocytes.

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Authors and Affiliations

  1. Abt. Exp. Medizin Martinsried, Max-Planck-Institut für Biochemie, Germany

    R. Scherer

  2. Dermatologische Klinik und Poliklinik der Universität München, Germany

    B. Kern & O. Braun-Falco

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  1. R. Scherer
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  2. B. Kern
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  3. O. Braun-Falco
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Scherer, R., Kern, B. & Braun-Falco, O. The human peripheral lymphocyte—a model system for studying the combined effect of psoralen plus black light. Klin Wochenschr 55, 137–140 (1977). https://doi.org/10.1007/BF01490241

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  • DOI: https://doi.org/10.1007/BF01490241

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