Zusammenfassung
Ein Radioimmunoassay wurde entwickelt zum Nachweis von HBeAg und anti-HBe in Serum oder Serumfraktionen. HBe/sAg positives Serum, teilweise gereinigtes HBeAg, teilweise gereinigtes HBsAg und HBe/sAg negatives Kontrollserum wurden in Polyacrylamid polymerisiert und auf ihre Bindungsfähigkeit für125I-IgG (anti-HBe) untersucht. Nur Gele mit immobilisiertem HBeAg reagierten spezifisch mit anti-HBe. Die Spezifität der Bindung wurde gesichert durch Blockierungs-/Inhibitionsteste mit anti-HBe, HBeAg, HBsAg und negativen Kontrollseren. Der Radioimmunoassay ermöglicht den spezifischen und quantitativen Nachweis von HBeAg und anti-HBe, auch in Gegenwart von Detergentien und hohen Salzkonzentrationen.
Summary
A solid-phase radioimmunoassay was developed for the detection of HBeAg and anti-HBe in sera or serum fractions. HBe/sAg positive sera, partially purified HBeAg, partially purified HBsAg, and HBe/sAg negative sera were polymerized in polyacrylamide and compared for their ability to bind125I-IgG (anti-HBe). Only gels containing HBeAg reacted specifically with the iodinated antibody. The specificity of the binding was confirmed by blocking and inhibition tests using anti-HBe, HBeAg, HBsAg, and negative control sera. The radioimmunoassay allows the specific and quantitative detection of HBeAg and anti-HBe even in the presence of detergents and high salt concentrations.
Abbreviations
- HBsAg:
-
hepatitis B surface antigen
- HBeAg:
-
hepatitis Be antigen
- HBe/sAg:
-
hepatitis Be antigen and surface antigen
- anti-HBe:
-
antibody to hepatitis Be antigen
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Blum, H.E., Dölken, G. & Gerok, W. Solid-phase radioimmunoassay for hepatitis be antigen (HBeAg). Klin Wochenschr 57, 1129–1132 (1979). https://doi.org/10.1007/BF01481494
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DOI: https://doi.org/10.1007/BF01481494