Summary
To investigate the effects of fluid expansion on endogenous atrial natriuretic peptide (ANP) and cyclic 3′,5′-guanosine monophosphate (cGMP), four male volunteers were studied before, during and after intravasal volume loading. Volume expansion was performed by intravenous infusion of 2,000 ml isotonic saline solution within 30 min. Mean plasma ANP levels increased 2.5-fold from 31.2 pg/ml to 81.7 pg/ml 40 min after the start of infusion. Plasma cGMP levels paralleled the rise in ANP, shwoing a mean cGMP increment from 2.7 pmol/ml to a maximum of 8.2 pmol/ml. Both ANP and cGMP levels were back to basal levels 120 min after termination of the infusion. Stimulation of endogenous ANP release by volume loading suggests that ANP is involved in the regulation of fluid homeostasis in man. The parallel rise in plasma cGMP levels supports the idea that cGMP is a mediator for the effects of ANP.
Similar content being viewed by others
Abbreviations
- ANP:
-
atrial natriuretic peptid
- cGMP:
-
cyclic 3′,5′-guanosine monophosphate
- PRA:
-
plasma renin activity
References
Burnett JC, Granger JP, Opgenorth TJ (1984) Effects of synthetic atrial natriuretic factor on renal function and renal release. Am J Physiol 247:F863-F866
Currie MG, Geller DM, Cole BR, Boylan JG, YuSheng W, Holmberg SW, Needleman P (1983) Bioactive cardiac substances: potent vasorelaxant activity in mammalian atria. Science 221:71–73
De Bold AJ, Borenstein HB, Veress AT, Sonnenberg H (1981) A rapid and potent natriuretic response to intravenous injection of atrial myocardial extract in rats. Life Sci 28:89–94
Garcia R, Cantin M, Thibault G, Ong H, Genest J (1982) Relationship of specific granules to the natriuretic and diuretic activity of rat atria. Experientia 38:1071–1073
Gerzer R, Tremblay J, Pang SC, DeLean A, Hamet P (1985) Atrial natriuretic factor activates mammalian particulate guanylate cyclase. Fed Proc 44:698
Gerzer R, Witzgall H, Tremblay J, Gutkowska J, Hamet P. Rapid increase in plasma and urinary cyclic GMP after bolus injection of atrial natriuretic factor in man. J Clin Endocrinol Metab (in press)
Graber SE, Hellerqvist CG (1982) Direct evidence that bacterial lipopolysaccharides elevate cyclic GMP levels in rat fetal liver cells. J Biol Chem 257:5414–5419
Hamet P, Tremblay J, Thibault G, Garcia R, Cantin M, Genest J (1983) Effects of atrial natriuretic factor on metabolism of cyclic GMP. The Endocrine Society 65:289
Hamet P, Tremblay J, Pang SC, Garcia R, Thibault G, Gutowska J, Cantin M, Genest J (1984) Effect of native and synthetic atrial natriuretic factor on cyclic GMP. Biochem Biophys Res Commun 123:515–527
Hirata Y, Tomita M, Yoshimi H, Ikeda M (1984) Specific receptors for atrial natriuretic factor (ANF) in cultured vascular smooth muscle cells of rat aorta. Biochem Biophys Res Commun 125:562–568
Kangawa K, Matsuo H (1984) Purification and complete amino acid sequence ofα-human atrial natriuretic polypeptide (α-hANP). Biochem Biophys Res Commun 118:131–39
Kangawa K, Fukuda A, Matsuo H (1985) Structural identification ofβ- andγ-human atrial natriuretic polypeptides. Nature 313:397–400
Kuribayashi T, Nakazato M, Tanaka M, Nagamine M, Kurihara T, Kangawa K, Matsuo H (1985) Renal effects of human alpha-atrial natriuretic polypeptide. N Engl J Med 312:1456–59
Lang RE, Thoelken H, Ganten D, Luft FC, Ruskoaho H, Unger Th (1985) Atrial natriuretic factor- a circulating hormone stimulated by volume loading. Nature 314:264–266
Maack T, Marion DM, Camargo MJ, Kleinert HD, Laragh JH, Vaughan ED, Atlas SA (1984) Effects of auriculin (Atrial Natriuretic Factor) on blood pressure, renal function and the renin-aldosterone system in dogs. Am J Med 77:1069–1075
Maki M, Paramentier M, Inagami T (1984) Cloning of genomic DNA for human atrial natriuretic factor. Biochem Biophys Res Commun 125:797–802
Oikawa S, Imai M, Ueno A, Ueno A, Tanaka S, Noguchi T, Nakazato H, Kangawa K, Fukuda A, Matsuo H (1984) Cloning and sequence analysis of cDNA encoding a precursor for human atrial natriuretic polypeptide. Nature 309:724–26
Rascher W, Tulassay T, Lang RE (1985) Atrial natriuretic peptide in plasma of volume overloaded children with chronic renal failure. Lancet ii:303–305
Richards AM, Nicholls MG, Ikram H, Webster MWI, Yandle TG, Espiner EA (1985) Renal, haemodynamic and hormonal effects of human alpha atrial natriuretic peptide in healthy volunteers. Lancet 1:545–549
Seidah NG, Lazure C, Chretien M, Thibault G, Garcia R, Cantin M, Genest J, Nutt RF, Brady SF, Lyle TA, Paleveda WJ, Colton CO, Ciccarone TM, Veber DF (1984) Amino acid sequence of homologous rat atrial peptides: Natriuretic activity of native and synthetic forms. Proc Natl Acad Sci USA 81:2640–2644
Steiner AL, Parker CW, Kipnis DM (1972) Radioimmunoassay for cyclic nucleotides I. Preparation of antibodies and iodinated cyclic nucleotides. J Biol Chem 247:1106–1113
Tremblay J, Gerzer R, Vinay P, Pang SC, Beliveau R, Hamet P (1985) The increase of cGMP by atrial natriuretic factor correlates with the distribution of particulate guanylate cyclase. FEBS Lett 181:17–22
Waldman SA, Rapoport RM, Murad F (1984) Atrial natriuretic factor selectively activates particulate guanylate cyclase and elevates cyclic GMP in rat tissues. J Biol Chem 259:14332–14334
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Weil, J., Lang, R.E., Suttmann, H. et al. Concomitant increase in plasma atrial natriuretic peptide and cyclic GMP during volume loading. Klin Wochenschr 63, 1265–1268 (1985). https://doi.org/10.1007/BF01738451
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01738451