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Die Bedeutung der Leukotriene bei chronischen Atemwegserkrankungen im Kindesalter

The role of leukotrienes in children with chronic airway diseases

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Summary

The role of lipoxygenase products was studied in children suffering from chronic diseases of the lung. Leukotrienes C4, D4, E4 and B4 were measured by high performance liquid chromatography (HPLC) and a specific radioimmunoassay (RIA) for C4. Elevated levels (up to 40 ng/ml), especially for leukotriene E4, were found in plasma of asthmatic and bronchitic patients (leukotriene C4 concentrations varied between 0.05 and 40 ng/ml, mean 4.9±7.8 ng/ml). In healthy donors the concentrations were below the detection limits of HPLC, leukotriene C4 ranging between 5±4 ng/ml (RIA data). The conversion of leukotriene C4 to D4 and E4 was observed by incubating the samples with synthetic leukotriene C4. The half-life of leukotriene C4 in plasma varied greatly, ranging from <12 min to 72 min (mean 39±16 min). Bronchial lavages yielded leukotriene C4 concentrations of 0.2 to 7 ng. Leukotriene E4 was detected in 10 of 41 cases. Conversion of leukotriene C4 did not occur in 50% of all cases, but was regularly observed in putrid lavages. These data suggest that leukotrienes play an important role in allergic and infectious lung diseases.

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Abbreviations

BSA:

Rinderserumalbumin

ECF:

Eosinophilchemotaktischer Faktor

ELISA:

Enzyme linked immunosorbent assay

GSH:

Glutathion

Glu:

Glutamin

Gly:

Glycin

5-HETE:

5-Hydroxyeicosatetraensäure

5-HPETE:

5-Hydroperoxyeicosatetraensäure

HPLC:

Hochleistungsflüssigkeits-chromatographie

LT:

Leukotrien

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Authors and Affiliations

  1. Medizinische Mikrobiologie und Immunologie, Arbeitsgruppe Infektabwehrmechanismen, Ruhr Universität Bochum, Germany

    W. Schönfeld, M. Köller, J. Knöller, W. Müller, H. v.d. Hardt & W. König

  2. Kinderklinik, Medizinische Hochschule Hannover, Germany

    W. Müller & H. v.d. Hardt

Authors
  1. W. Schönfeld
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  2. M. Köller
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  3. J. Knöller
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  4. W. Müller
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  5. H. v.d. Hardt
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  6. W. König
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Additional information

Die Autoren bedanken sich für die großzügige Förderung durch die Stiftung Volkswagenwerk, die diese Arbeit im Rahmen der Förderung „Das chronisch kranke Kind“ ermöglicht hat (Bewilligung der VW-Stiftung: I-37892)

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Schönfeld, W., Köller, M., Knöller, J. et al. Die Bedeutung der Leukotriene bei chronischen Atemwegserkrankungen im Kindesalter. Klin Wochenschr 64, 257–264 (1986). https://doi.org/10.1007/BF01711931

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  • DOI: https://doi.org/10.1007/BF01711931

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