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Einbau von15N-Glyzin in VLDL und LDL: In-vivo-Synthese von Apolipoprotein B beim Menschen postabsorptiv und im Fastenzustand

Incorporation of15N-glycine into VLDL and LDL: In vivo synthesis of apolipoprotein B in post-absorptive and fasting individuals

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Summary

In vivo synthesis of apolipoprotein B 100 (ApoB) was recently determined in man using stable isotopes. With this procedure we analyzed (1) the effect of fasting on synthesis of ApoB from very low density lipoprotein (VLDL) and (2) tracer enrichment in low density lipoprotein (LDL).

After a 36-hour fasting period and in the post-absorptive state 4 healthy subjects were given a priming dose (8.7 µmol/kg) of15N glycine followed by a constant infusion (10 µmol/kg/h for 8 h) to achieve 5% tracer enrichment in the plasma pool of glycine. The K-values, i.e. fractional synthetic rates/hr of ApoB from VLDL were 0.53±0.26 vs. 0.43±0.16 (p>0.05). Tracer enrichment in ApoB from LDL at the end of the infusions was 0.19% vs. 1.46% in ApoB from VLDL.

The results indicate that (1) in young healthy postabsorptive individuals about 40% of ApoB from VLDL in plasma is synthesized per hour, (2) fasting does not materially affect fractional ApoB synthesis and (3) at 5%15N enrichment in plasma glycine, tracer enrichment in ApoB from LDL is at the lower limit of detection for the procedure employed.

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Abbreviations

ApoB:

Apolipoprotein B 100

LDL:

low density lipoprotein

VLDL:

very low density lipoprotein

ISV:

Ionenstrom-Verhältnis

SIM:

selected ion monitoring

APZ:

Atom-Prozent-Zunahme

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Authors and Affiliations

  1. Medizinische Klinik, Abteilung für Gastroenterologie und Endokrinologie, Universität Göttingen, Germany

    P. Schauder & G. Schäfer

  2. School of Medicine, Metabolism Division, Washington University, St. Louis, MO, USA

    J. Arends & D. M. Bier

  3. Institut für experimentelle Ernährung, Erlangen

    K. Langer

Authors
  1. P. Schauder
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  2. J. Arends
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  3. G. Schäfer
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  4. K. Langer
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  5. D. M. Bier
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Schauder, P., Arends, J., Schäfer, G. et al. Einbau von15N-Glyzin in VLDL und LDL: In-vivo-Synthese von Apolipoprotein B beim Menschen postabsorptiv und im Fastenzustand. Klin Wochenschr 67, 280–285 (1989). https://doi.org/10.1007/BF01892895

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  • DOI: https://doi.org/10.1007/BF01892895

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