Skip to main content
SpringerLink
Log in

Decrease of the inhibition of lipid peroxidation by glutathione-dependent system in erythrocytes of non-insulin dependent diabetics

  • Originalien
  • Published:
Klinische Wochenschrift Aims and scope Submit manuscript

Summary

The inhibition of lipid peroxidation of erythrocyte membranes by glutathione-dependent protection was studied in patients with non-insulin dependent diabetes mellitus. Incubation of red cells from diabetics with 1.5 mM t-butyl hydroperoxide resulted in a lipid peroxidation increase greater than that of normal controls. Glutathione-dependent and glutathione-independent protection against oxidative damage was examined using an artificial system, in which erythrocyte ghosts were incubated with t-butyl peroxide and dialysed hemolysate in the presence or the absence of 2 mM glutathione. The glutathione-dependent protection of hemolysate from diabetics was approximately 70% of that from normal controls.

The results suggest that decrease in glutathione-dependent protection against lipid peroxidation, along with decrease in glutathione levels, increases oxidative damage in erythrocyte membranes taken from diabetic patients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

GSH:

reduced form of glutathione

GSSG:

glutathione disulfide

TCA:

trichloracetic acid

SOD:

superoxide dismutase

GPX:

glutathione peroxidase

MDA:

malondialdehyde

References

  1. Arrick BA, Nathan CF, Griffth OW, Cohn ZA (1982) Glutathione depletion sensitizes tumor cells to oxidative cytolysis. J Biol Chem 257:1231–1237

    Google Scholar 

  2. Beauchamp E, Fridovich I (1971) Superoxide dismutase: improved assay and assay applicable to acrylamide gels. Anal Biochem 44:276–287

    Google Scholar 

  3. Beutler E, West C, Blume RV (1976) The removal of leukocytes and platelets from whole blood. J Lab Clin Med 88:328–333

    Google Scholar 

  4. Beutler E (1984) Red Cell Metabolism: A Manual of Biochemical Methods, 3rd ed. Grune & Stratton, New York

    Google Scholar 

  5. Bieri JG, Anderson AA (1960) Peroxidation of lipids in tissue homogenates as related to vitamin E. Arch Biochem Biophys 90:105–110

    Google Scholar 

  6. Bono A, Gaimi G, Catania A, Sarno A, Pandorfo L (1987) Red cell peroxide metabolism in diabetes mellitus. Horm Metabol Res 19:264–266

    Google Scholar 

  7. Burk RF (1983) Glutathione-dependent protection by rat liver microsomal protein against lipid peroxidation. Biochim Biophys Acta 757:21–28

    Google Scholar 

  8. Coetzer T, Zail S (1980) Membrane protein complexes in GSH-depleted red cells. Blood 56:159–167

    Google Scholar 

  9. Davies KJA, Goldberg AL (1987) Proteins damaged by oxygen radicals are rapidly degraded in extracts of red blood cells. J Biol Chem 262:8227–8234

    Google Scholar 

  10. Fujii S, Dale GL, Beutler E (1984) Glutathione-dependent protection against oxidative damage of the human red cell membrane. Blood 63:1096–1101

    Google Scholar 

  11. Haenen GRMM, Bast A (1983) Protection against lipid peroxidation by a microsomal glutathione-dependent labile factor. FEBS Lett 159:24–28

    Google Scholar 

  12. Kaji H, Kurasaki K, Ito K, Saito K, Niioka T, Kojima Y, Ohsaki Y, Ide H, Tsuji M, Kondo T, Kawakami Y (1985) Increased lipid peroxide value and glutathione peroxidase activity in blood of type 2 (non-insulin-dependent) diabetic woman. Klin Wochenschr 63:765–768

    Google Scholar 

  13. Kappus H (1985) Lipid peroxidation: mechanisms, analysis, enzymology and biological relevance. In: Sies H (ed) Oxidative Stress. Academic Press, New York, pp 273–310

    Google Scholar 

  14. Kondo T, Dale GL, Beutler E (1980) Glutathione transport by inside-out vesicles from human erythrocytes. Proc Natl Acad Sci USA 77:6359–6362

    Google Scholar 

  15. Kondo T, Kawakami Y, Taniguchi N, Beutler E (1987) Glutathione disulfide-stimulated Mg2+-ATPase of human erythrocyte membranes. Proc Natl Acad Sci USA 84:7373–7377

    Google Scholar 

  16. McCay PB, Gibson DD, Hornbrook KR (1981) Glutathione-dependent inhibition of lipid peroxidation by a soluble, heat-labile factor not glutathione peroxidase. Fed Proc 40:199–205

    Google Scholar 

  17. Owens CWI, Belcher RV (1965) A colorimetric micro-method for the determination of glutathione. Biochem J 904:705–711

    Google Scholar 

  18. Prchal J, Srivastava SK, Beutler E (1975) Active transport of GSSG from reconstituted erythrocyte ghosts. Blood 46:111–117

    Google Scholar 

  19. Uzel N, Sivas A, Uysal M, Oz H (1987) Erythrocyte lipid peroxidation and glutathione peroxidase activities in patients with diabetes mellitus. Horm Metabol Res 19:89–90

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. First Department of Medicine, Hokkaido University School of Medicine, Sapporo, Japan

    Y. Fujiwara, T. Kondo, K. Murakami & Y. Kawakami

Authors
  1. Y. Fujiwara
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Kondo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Murakami
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. Kawakami
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fujiwara, Y., Kondo, T., Murakami, K. et al. Decrease of the inhibition of lipid peroxidation by glutathione-dependent system in erythrocytes of non-insulin dependent diabetics. Klin Wochenschr 67, 336–341 (1989). https://doi.org/10.1007/BF01741388

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01741388

Key words

Search

Navigation