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Generation of leukotriene B4 by hemodialyzer membranes: A novel index of biocompatibility

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Summary

Leukotriene B4 (LTB4) plays an important role in acute and chronic inflammatory and hypersensitive reactions. We studied the time course of LTB4 biosynthesis in whole blood in 18 patients with end-stage renal failure maintained on regular hemodialysis with two different membranes, cuprophane and polyacrylonitrile (AN 69). The basal levels of LTB4 from dialysis patients did not differ significantly from a normal control group. Compared to predialytic values, the cuprophane membrane caused a maximal release of LTB4 by a factor of about 4.5 (p<0.01) within the first 10 to 20 minutes. Thereafter the level fell and returned to baseline range at the end of the hemodialysis session. With the use of the AN 69 membrane no significant increase of LTB4 could be demonstrated. The changes in LTB4 concentration showed a close temporal correlation to the alterations in white blood cell count. We conclude that (1) LTB4 is a biologically important mediator of neutrophil activation during hemodialysis, and (2) LTB4 may be a sensitive marker of biocompatibility in vivo.

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Abbreviations

LTB4 :

Leukotriene B4

5-HETE:

5-Hydroxyeicosatetraenoic acid

References

  1. Basile C, Druecke T (1989) Dialysis membrane biocompatibility. Nephron 52:113–118

    Google Scholar 

  2. Betz M, Haensch GM, Rauterberg EW, Bommer J, Ritz E (1984) Cuprammonium membranes stimulate Interleukin 1 release and arachidonic acid metabolites in monocytes in the absence of complement. Kidney Int 34:67–73

    Google Scholar 

  3. Clancy RM, Dahinden CA, Hugli TE (1985) Complement-mediated arachidonate metabolism. Progr Biochem Pharmacol 20:120–131

    Google Scholar 

  4. Craddock P, Fehr E, Dalmasso A, Brigham K, Jacob H (1977) Hemodialysis leukopenia: pulmonary leukostasis resulting from complement activation by dialysis cuprophane membranes. J Clin Invest 59:879–888

    Google Scholar 

  5. De Broe ME, De Baker WA (1989) Pathophysiology of hemodialysis-associated hypoxemia. Adv Nephrol 18:297–316

    Google Scholar 

  6. Foidart JB, Davin JC, Malaise M, Saint-Remy M, Mahieu P (1985) Stimulation of platelet lipoxygenase during hemodialysis. Kidney Int 33 [Suppl 24]:80–81

    Google Scholar 

  7. Haag-Weber M, Hable M, Schollmeyer P, Hörl WH (1989) Metabolic response of neutrophils to uremia and dialysis. Kidney Int [Suppl 27]:293–298

    Google Scholar 

  8. Hakim RA (1985) Biocompatibility issues in hemodialysis. Proc Eur Dial Transplant 22:163–170

    Google Scholar 

  9. Hammerschmidt DE, Goldberg R, Raij L, Kay NE (1985) Leukocyte abnormalities in renal failure and hemodialysis. Sem Nephrol 5:91–103

    Google Scholar 

  10. Henderson LW, Chenoweth D (1987) Biocompatibility of artificial organs: an overview. Blood purification 5:100–111

    Google Scholar 

  11. Hoenich NA, Woffinden C, Ward MU (1989) Dialyzers. In: Maher JF (ed) Replacement of renal function by dialysis. Klüwer, Dodrecht, Holland, pp 144–180

    Google Scholar 

  12. Hörl WH, Jochum M, Heidland A, Fritz H (1983) Release of granulocyte proteinases during hemodialysis. Am J Nephrol 3:213–217

    Google Scholar 

  13. Jörres A, Richter D, Gahl M, Mahiout A (1990) Leukotriene B4 in patients on hemodialysis with cellulosic membranes. 27th Congress of the European Dialysis and Transplant Association, Vienna, p 163 (abstract)

  14. Kolb G, Fischer M, Schoenemann H, Bathke K, Höffken H, Müller T, Lange H, Joseph K, Havemann K (1989) Effect of cuprophane, hemophane and polysulphone membranes on the oxidative metabolism, degranulation reaction, enzyme release and pulmonary sequestration of granulocytes. Contrib Nephrol 74:10–21

    Google Scholar 

  15. Lewis RA, Austen K, Soberman RJ (1990) Mechanisms of disease. Leukotrienes and other products of the 5-lipoxygenase pathway — biochemistry and relation to pathobiology in human disease. N Engl JM 323:645–655

    Google Scholar 

  16. Lewis SL, Van Epps DE (1987) Neutrophil and monocyte alterations in chronic dialysis patients. Am J Kidney Dis 9:381–395

    Google Scholar 

  17. Linke RP, Hampl H, Lobeck H, Ritz E, Bommer J, Waldherr R, Eulitz M (1989) Lysine-specific cleavage ofβ 2-microglobulin in amyloid deposits associated with hemodialysis. Kidney Int 36:675–681

    Google Scholar 

  18. Mahiout A, Jörres A, Hiss R, Meinhold H, Kessel M (1987) Effect of blood-dialyzer interaction on prostaglandins in uremic patients and in healthy man. Nephrol Dial Transplant 2:546–550

    Google Scholar 

  19. McMillan RM, Foster SJ (1988) Leukotriene B4 and inflammatory disease. Agents and Actions 24:114–119

    Google Scholar 

  20. Nacchache PH, Shaafi RI (1983) Arachidonic acid, leukotriene B4 and neutrophil activation. Ann NY Acad Sci 414:125–139

    Google Scholar 

  21. Newcombe DS (1988) Leukotrienes: regulation of biosynthesis, metabolism and bioactivity. J Clin Pharmacol 28:530–549

    Google Scholar 

  22. Remy PH, Hornych A, Luong N, Aumont J, Bariety J (1990) Biocompatibility of dialysis membranes: the role of leukotriences. 27th Congress of the European Dialysis and Transplant Association, Vienna, p 163 (abstract)

  23. Strasser Th, Schiffl H (1990) Leukotriene B4 is involved in hemodialysis-associated neutropenia. Biochim Biophys Acta 1064:326–329

    Google Scholar 

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  1. Medizinische Klinik, Klinikum Innenstadt der Universität, München

    T. Strasser & H. Schiffl

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  1. T. Strasser
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  2. H. Schiffl
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Strasser, T., Schiffl, H. Generation of leukotriene B4 by hemodialyzer membranes: A novel index of biocompatibility. Klin Wochenschr 69, 808–812 (1991). https://doi.org/10.1007/BF01744274

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  • DOI: https://doi.org/10.1007/BF01744274

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