Skip to main content
SpringerLink
Log in

Circulating adhesion molecules ICAM-1, VCAM-1 and E-selectin in systemic vasculitis : marked differences between Wegener's granulomatosis and systemic lupus erythematosus

  • Original Article
  • Published:
The clinical investigator Aims and scope Submit manuscript

Abstract

Inflammatory systemic disorders with renal tissue damage require the adherence of polymorphonuclear leukocytes to the endothelium, which is mediated by cell surface adhesion molecules. This study measured the serum concentrations of circulating adhesion molecules [intercellular adhesion molecule 1 (cICAM-1), vascular cell adhesion molecule 1 (cVCAM-1), cE-selectin] by sandwich enzyme-linked immunosorbent assay in Wegener's granulomatosis (n = 25) and systemic lupus erythematosus (n = 50). Active Wegener's granulomatosis with cellular crescent formation was associated with significantly raised cICAM-1 levels, an elevated histological activity index, and a high rate of hemodialysis in comparison to Wegener's granulomatosis with fibrocellular crescents or lupus nephritis. The activity index and cICAM-1 levels can be used as parameters to predict renal outcome in Wegener's granulomatosis. cVCAM-1 levels in Wegener's granulomatosis were significantly higher in patients with hemodialysis compared to those without hemodialysis. In lupus nephritis only cVCAM-1, not cICAM-1 or cE-selectin, were significantly higher (P < 0.01) than controls. cVCAM-1 levels in both diseases were significantly higher than in controls. Only in Wegener's granulomatosis did cICAM-1 and cVCAM-1 levels decrease with clinical remission; in systemic lupus erythematosus the levels remained unchanged. cE-selectin levels were not significantly elevated in systemic vasculitis or collagen disease although individual patients revealed high serum concentrations. These data suggest that levels of circulating adhesion molecules reflect different pathophysiological processes in systemic vasculitis and collagen disease and may be used as markers of disease activity and renal outcome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AI:

activity index

ANA:

anti-nuclear antibody

ANCA:

anti-neutrophil cytoplasmic autoantibody

cICAM-1:

circulating intercellular adhesion molecule-1

cVCAM-1:

circulating vascular cell adhesion molecule-1

CI:

chronicity index

IFT:

immunofluorescence test

LFA-1:

lymphocyte function-associated antigen 1

LN:

lupus nephritis

RPGN:

rapidly progressive glomerulonephritis

SLE:

systemic lupus erythematosus

WG:

Wegener's granulomatosis

References

  1. Adams DH, Mainolfi E, Burra P,Neuberger JM, Ayres R, Elias E, Rothlein R (1992) Detection of circulating intercellular adhesion molecule-1 in chronic liver diseases. Hepatology 16:810–814

    Google Scholar 

  2. Alpers CE (1993) Expression of vascular cell adhesion molecule-1 in kidney allograft rejection. Kidney Int 44:805–816

    Google Scholar 

  3. Austin HA, Muenz LR, Joyce KM, Antonovych TA, Kullick MA, Klippel JH, Decker JL, Balow JE (1983) Prognostic factors in lupus nephritis: contribution of renal histological data. Am J Med 75:382–391

    Google Scholar 

  4. Banks RE, Gearing AJH, Hemingway IK, Norfolk DR, Perren TJ, Selby PJ (1993) Circulating intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1 (VCAM-1) in human malignancies. Br J Cancer 68:122–124

    Google Scholar 

  5. Becker JC, Dummer R, Hartmann AA, Burg G, Schmidt RE (1991) Shedding of ICAM-1 from human melanoma cell lines induced by IFN-gamma and tumor necrosis factor alpha. J Immunol 147:4398–4401

    Google Scholar 

  6. Bevilacqua MP, Pober JS, Mendrick DL, Cotran RS, Gimbrone MA (1987) Identification of an inducible endothelial-leukocyte adhesion molecule. Proc Natl Acad Sci 84:9238–9243

    Google Scholar 

  7. Bevilacqua MP, Stengelin S, Gimbrone MA, Seed B (1989) Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science 243:1160–1167

    Google Scholar 

  8. Cush JJ, Rothlein R, Lindsley HB, Mainolfi EA, Lipsky PE (1993) Increased levels of circulating intercellular adhesion molecule-1 in the sera of patients with rheumatoid arthritis. Arthritis Rheum 36:1098–1102

    Google Scholar 

  9. Dinther-Janssen ACHM van, Horst E, Koopman G, Newman W, Scheper RJ, Meijer CJLM, Pals ST (1991) The VLA4/VCAM-1 pathway is involved in lymphocyte adhesion to endothelium in rheumatoid synovium. J Immunol 147:4207–4210

    Google Scholar 

  10. Gans ROB, Kuizinga MC, Goldschmeding R, Assmann K, Huysmanns FTM, Gerlag PGG, Donker AJM, Hoorntje SJ (1993) Clinical features and outcome in patients with glomerulonephritis and antineutrophil cytoplasmic autoantibodies. Nephron 64:182–188

    Google Scholar 

  11. Gearing AJH, Hemmingway IK, Pigott R, Hughes J, Rees AJ, Cashman SJ (1992) Soluble forms of vascular adhesion molecules, E-selectin, ICAM-1 and VCAM-l:pathological significance. Annals NY Acad Sci 187:234–245

    Google Scholar 

  12. Hession C, Osborn L, Goff D, Chi-Rosso G, Vassallo C, Pasek M, Pittack C, Tizard R, Goelz S, McCarthy K (1990) Endothelial leukocyte adhesion molecule 1: direct expression cloning and functional interactions. Proc Natl Acad Sci 87:1673–1678

    Google Scholar 

  13. Hill PA, Lan HY, Nikolic-Paterson DJ, Atkins RC (1994) The ICAM-1/LFA-1 interaction in glomerular leukocytic accumulation in anti-GBM glomerulonephritis. Kidney Int 45:700–708

    Google Scholar 

  14. Koch AE, Turkiewicz W, Harlow LA, Pope RM (1993) Soluble E-selectin in arthritis. Clin Immunol Immunopathol 69:29–35

    Google Scholar 

  15. Leavitt RY, Fauci AS, Bloch DA, Michel BA, Hunder GG, Arend WP, Calabrese LH, Fries JF, Lie JT, Lightfood RW, Masi AT, McShane DJ, Mills JA, Stevens MB, Wallace SL, Zvaifler NJ (1990) Criteria for the classification of Wegener's granulomatosis. American College of Rheumatology. Arthritis Rheum 33:1101

    Google Scholar 

  16. Linker-Israeli M, Deans RJ, Wallace DJ, Prehn J, Ozeri-Chen T, Klinenberg JR (1991) Elevated levels of endogenous Il-6 in systemic lupus erythematosus. A putative role in pathogenesis. J Immunol 147:117–123

    CAS  PubMed  Google Scholar 

  17. Lobb R, Chi-Rosso G, Leone DR, Rosa MD, Bixler S, Newmann BM, Luhowskyj S, Benjamin C, Douglas I, Goelz SE (1991) Expression and functional characterization of a soluble form of endothelial-leukocyte adhesion molecule 1. J Immunol 147:124–130

    Google Scholar 

  18. Mason JC, Kapahi P, Haskard DO (1993) Detection of increased levels of circulating intercellular adhesion molecule 1 in some patients with rheumatoid arthritis but not in patients with systemic lupus erythematosus. Arthritis Rheum 36:519–527

    Google Scholar 

  19. Müller GA, Markovic-Lipkovski J, Miiller CA (1991) Intercellular adhesion molecule-1 expression in human kidneys with glomerulonephritis. Clin Nephrol 36:203–208

    Google Scholar 

  20. Newman W, Dawson Beall L, Carson CW,Hunder GG, Graben N, Randhawa ZI, Gopal TV, Wiener-Kronish J, Matthay MA (1993) Soluble E-selectin is found in supernatants of activated endothelial cells and is elevated in the serum of patients with septic shock. J Immunol 150:644–654

    Google Scholar 

  21. Nishikawa K, Guo YJ, Miyasaka M, Tamatani T, Collins AB, Sy MS, MyCluskey RT, Andres G (1993) Antibodies to intercellular adhesion molecule 1/lymphocyte function-associated antigen 1 prevent crescent formation in rat autoimmune glomerulonephritis. J Exp Med 177:667–677

    Google Scholar 

  22. Osborn L, Hession C, Tizard R, Vassallo C, Luhowskyj S, Chi-Rosso G, Lobb R (1989) Direct expression cloning of vascular cell adhesion molecule l, a cytokine-induced endothelial protein that bind to lymphocytes. Cell 59:1203–1211

    Google Scholar 

  23. Pigott R, Dillon LP, Hemingway IH, Gearing AJH (1992) Soluble forms of E-selectin, ICAM-1 and VCAM-1 are present in the supernatants of cytokine-activated endothelial cells. Biochem Biophys Res Commun 187:584–589

    Google Scholar 

  24. Rothlein R, Mainolfi EA, Czajkowski M, Marlin S (1991) A form of circulating ICAM-1 in human serum. J Immunol 147:3788–3793

    Google Scholar 

  25. Schmitt WH, Heesen C, Csernok E, Gross WL (1992) Elevated serum levels of soluble interleukin-2 receptor (sIl-2R) in Wegener's granulomatosis (WG): association with disease activity. Arthritis Rheum 35:108–110

    Google Scholar 

  26. Seron D, Cameron JS, Haskard DO (1991) Expression of VCAM-1 in the normal and diseased kidney. Nephrol Dial Transplant 6:917–922

    Google Scholar 

  27. Springer TA (1990) Adhesion receptors of the immune system. Nature 346:425–434

    Google Scholar 

  28. Stockenhuber F, Kramer G, Schenn G, Pee M, Gnant M, Steininger R, Balcke P, Mühlbacher F (1993) Circulating ICAM-1: novel parameter of renal graft rejection. Transplant Proc 25:919–920

    Google Scholar 

  29. Tan EM, Cohen AS, Fries JF, Masi AT, McShane DJ, Rothfield NF, Schaller JG, Talal N, Winchester RJ (1982) The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheumat 25:1271–1277

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Medizinische Klinik II, Rheinisch-Westfälische Technische Hochschule Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany

    C. Mrowka & H. G. Sieberth

Authors
  1. C. Mrowka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. G. Sieberth
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Correspondence to: C. Mrowka

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mrowka, C., Sieberth, H.G. Circulating adhesion molecules ICAM-1, VCAM-1 and E-selectin in systemic vasculitis : marked differences between Wegener's granulomatosis and systemic lupus erythematosus. Clin Investig 72, 762–768 (1994). https://doi.org/10.1007/BF00180543

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00180543

Key words

Search

Navigation