Abstract
Microdialysis has been proven to be a valuable tool to study in vivo release of various neurotransmitters in the rat brain. Recently we demonstrated for the first time the release of neurotrophic bioactivity in the brains of awake rats. Neurotrophic factors, however, exist in extremely low concentrations in the brain compared to neurotransmitters, rendering their detection particularly difficult. This review summarizes knowledge about the use of microdialysis for the detection of neurotrophic bioactivity, its limits, and its problems.
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Abbreviations
- BDNF :
-
Brain-derived neurotrophic factor
- EIA :
-
Enzyme immunoassay
- NGF :
-
Nerve growth factor
- NT-3:
-
Neurotrophin-3
References
Zetterströom T, Sharp T, Marsden C and Ungerstedt U (1983) In vivo measurement of dopamine and its metabolites by intracerebral dialysis: changes after d-amphetamine J Neurochem 41:1769–1773
Di Chiara G (1990) In vivo brain dialysis of neurotransmitters. Trends Pharmacol Sci 11:116–121
Kalen P, Nilsson OG, Cenci MA, Rosengren E, Lindvall O, Björklund A (1990) Intracerebral microdialysis as a tool to monitor transmitter release from grafted cholinergic and monoaminergic neurons. J Neurosci Methods 34:107–115
Kendrick KM (1989) Use of microdialysis in neuroendocrinology. Methods Enzymol 168:182–205
Levine JE, Powell KD (1989) Microdialysis for measurement of neuroendocrine peptides. Methods Enzymol 168:166–181
Benveniste H (1989) Brain microdialysis. J Neurochem 52: 1667–1679
Humpel C, Lindqvist E, Söderström S, Kylberg A, Ebendal T, Olson L (1995) Monitoring release of neurotrophic activity in the brains of awake rats. Science 269:552–554
Ruggeri M, Zoli M, Grimaldi R, Ungerstedt U, Eliasson A, Agnati LF, Fuxe K (1990) Aspects ofneural plasticity in the central nervous system. III. Methodological studies on the microdialysis technique. Neurochem Int 16:427–435
Benveniste H, Diemer NH (1987) Cellular reactions to implantation of a microdialysis tube in the rat hippocampus. ActaNeuropathol 74:234–238
Shuaib A, Xu K, Crain B, Siren AL, Feuerstein G, Hallenbeck J, Dais JNv (1990) Assessment of damage from implantation of microdialysis probes in the rat hippocampus with silver degeneration staining. Neurosci Lett 112:149–154
Benveniste H, Drejer J, Schousboe A, Diemer N (1987) Regional cerebral glucose phosphorylation and blood flow after insertion of a microdialysis fiber through the dorsal hippocampus in the rat. J Neurochem 49 49:729–73
Nieto-Sampedro M, Lewis ER, Cotman CW, Manthorpe M, Skaper SD, Barbin G, Longo FM, Varon S (1982) Brain injury causes a time-dependent increase in neuronotrophic activity at the lesion side. Science 217:860–861
Finklestein SP, Apostolides PJ, Caday CG, Prosser J, Philips MF, Klagsbrun M (1988) Increased basic fibroblast growth factor (bFGF) immunoreactivity at the site of focal brain wounds. Brain Res 460:253–259
Humpel C, Chadi G, Lippoldt A, Ganten D, Fuxe K, Olson L (1994) Increase of basic fibroblast growth factor (bFGF, FGF-2) messenger RNA and protein following implantation of a microdialysis probe into rat hippocampus. Exp Brain Res 98:229–237
Ballarin M, Ernfors P, Lindefors N, Persson H (1991) Hippocampal damage and kainic acid injection induce a rapid increase in mRNA for BDNF and NGF in the rat brain. Exp Neurol 114:35–43
Gall CM (1992) Regulation of brain neurotrophin expression by physiological activity. Trends Pharmacol Sci 13:401–403
Lindholm D, Castren E, Berzaghi M, Blöchl A, Thoenen H (1994) Activity-dependent and hormonal regulation of neurotrophin mRNA levels in the brain. Implications for neuronal plasticity. J Neurobiol 25:1362–1372
Lindvall O, Kokaia Z, Bengzon J, Elmer E, Kokaia M (1994) Neurotrophins and brain insults. Trends Neurosci 17:490–496
Ebendal T (1989) Use of collagen gels to bioassay nerve growth factor activity. In: Rush RA et al. (eds) Methods in the neurosciences: nerve growth factors, vol 12. John Wiley & Sons, New York, pp 81–94
Blöchl A, Thoenen H (1995) Characterization of nerve growth factor (NGF) release from hippocampal neurons: evidence for a constitutive and an unconventional sodium-dependent regulated pathway. Eur J Neurosci 7:1220–1228
Lindsay RM (1994) Neurotrophic growth factors and neurode-generative diseases: therapeutic potential of the neurotrophins and ciliary neurotrophic factor. Neurobiol Aging 15:249–251
Lindsay RM, Wiegand SJ Altar CA, DiStefano PS (1994) Neurotrophic factors: from molecule to man. Trends Neurosci 17:182–190
Hefti F (1994) Neurotrophic factor therapy for nervous system degenerative diseases. J Neurobiol 25:1418–1435
Tomac A, Lindqvist E, Lin LFH, Ögren SO, Young D, Hoffer BJ, Olson L (1995) Protection and repair of the nigrostriatal dopaminergic system by GDNF in vivo. Nature 373:335–339
Seiger A, Nordberg A, Von Holst H, Böckman L, Ebendal T, Alafuzoff I, Amberla K, Hartvig P, Herlitz A, Lilja A, Lundqvist H, Langström B, Meyerson B, Persson A, Viitanen M, Winblad B, Olson L (1993) Intracranial infusion of purifiednerve growth factor to an Alzheimer patient: the first attempt of a possible future treatment strategy. Behav Brain Res 57: 255–261
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Humpel, C., Ebendal, T. & Olson, L. Microdialysis: a way to study in vivo release of neurotrophic bioactivity: a critical summary. J Mol Med 74, 523–526 (1996). https://doi.org/10.1007/BF00204978
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DOI: https://doi.org/10.1007/BF00204978