Summary
Two strains of meat-type chickens which had been derived from the same genetic base, but were selected for high or low abdominal fat content, respectively, were analyzed for polymorphisms in the growth hormone gene (GH). A total of four DNA polymorphisms were identified, one at a SacI restriction site and three at MspI restriction sites. Restriction mapping indicated that all polymorphisms were in exons and/or introns and not in flanking regions of the gene. The incidence of GH polymorphisms was determined in 20 chickens from each strain and significant differences were observed for two of the four polymorphisms. Analysis by DNA fingerprinting using (CAC)5 as a probe indicated that the inbreeding coefficient was 0.1 in both strains and that random genetic drift was minimal. Thus, the selection for abdominal fat appears to have affected the frequency of alleles of the growth hormone gene. Whether this is the direct consequence of an altered growth hormone gene on fat metabolism or reflects linkage to an allele of a neighbouring gene remains to be determined.
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References
Ali S, Muller CR, Epplen JT (1986) DNA fingerprinting by oligonucleotide probes specific for simple repeats. Human Genet 74:239–243
Breier BH, Gluckman PB, McCutcheon SN, Davis SR (1989) Physiological responses to somatotropin in the ruminant. J Dairy Sci 74:20–34
Campbell RM, Scanes CG (1985) Lipolytic activity of purified pituitary and bacterially derived growth hormone on chicken adipose tissue in vitro. Proc Soc Exp Biol Med 180:513–516
Harvey S, Scanes CG, Ho we T (1977) Growth hormone effects on in-vitro adipose and liver tissue. Gen Comp Endocrinol 33:322–328
Jeffreys AJ, Morton DB (1987) DNA fingerprints of dogs and cats. Anim Genet 18:1–15
Kuhnlein U, Gavora JS, Spencer JL, Bernon DE, Sabour M (1989) Incidence of endogenous viral genes in two strains of White Leghorn chickens selected for egg production and susceptibility or resistance to Marek's disease. Theor Appl Genet 77:36–32
Kuhnlein U, Zadworny D, Dawe Y, Fairful RW, Gavora JS (1990) Assessment of inbreeding by DNA fingerprinting: development of a calibration curve using defined strains of chickens. Genetics 125:161–165
Leclercq B (1988) Genetic selection of meat-type chickens for low abdominal fat content. In: Leclercq B, Whitehead CC (eds) Leanness in domestic birds. Butterworth, London Boston Singapore Sydney Toronto Wellington, pp 25–40
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory handbook. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Scanes CG (1987) The physiology of growth, growth hormones and other growth factors in poultry. CRC Crit Rev Poultry Biol 1:51–105
Whitehead CC (1988) Selection for leanness in broilers using plasma lipoprotein concentration as a selection criterion. In: Leclercq B, Whitehead CC (eds) Leanness in domestic birds. Butterworth, London Boston Singapore Sydney Toronto Wellington, pp 41–57
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Communicated by C. Smith
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Fotouhi, N., Karatzas, C.N., Kuhnlein, U. et al. Identification of growth hormone DNA polymorphisms which respond to divergent selection for abdominal fat content in chickens. Theoret. Appl. Genetics 85, 931–936 (1993). https://doi.org/10.1007/BF00215031
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DOI: https://doi.org/10.1007/BF00215031