Abstract
A non-radioactive digoxigenin-labelled DNA method was used successfully to identify RFLP markers in 54 Triticum aestivum cv ‘Chinese Spring’ — Agropyron cristatum (2n=28, genome PPPP) P-genome monosomic addition lines. Southern analysis using a set of 14 DNA probes identifying each homoeologous chromosome arm, combined with two restriction enzymes HindIII and EcoRI, indicated that six A. cristatum chromosomes (1P, 2P, 3P, 4P, 5P and 6P) and five A. cristatum chromosome arms (2PS, 2PL, 5PL, 6PS and 6PL) have been individually added to the wheat genome. The added chromosomes of three lines were Agropyron translocated chromosomes. It was also found that two addition plants possessed an Agropyron-wheat translocation. These results showed that RFLP analysis using the set of assigned wheat probes was a powerful tool in detecting and establishing homoeology of alien A. cristatum chromosomes, or arms, added to wheat, as well as in screening the alien addition material. The creation of the monosomic addition lines should be useful for the transfer of disease-resistance genes from A. cristatum to wheat.
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Ahmad F, Comeau A (1991) A new intergeneric hybrid between Triticum aestivum L. and Agropyron fragile (Roth) Candargy: variation in A. fragile for suppression of the wheat Ph-locus activity. Plant Breed 106:275–283
Cauderon Y (1966) Étude cytogénétique de l'évolution du matériel issu de croisement entre Triticum aestivum et Agropyron intermedium. Ann Amélior Plantes 16:43–70
Cauderon Y, Saigne B, Dauge M (1973) The resistance to wheat rusts of Agropyron intermedium and its use in wheat improvement. Proc 4th Int Wheat Genet Symposium, Columbia, Missouri, pp 401–407
Cauderon Y, Autran JC, Joudrier P, Kobrehel K (1978) Identification de chromosomes d'Agropyron intermedium impliqués dans la synthèse des gliadines, des α-amylases et des péroxydases à l'aide de lignées d'addition Blé x Agropyron. Ann Amélior Plantes 28:257–267
Charpentier A (1992) Production de lignées d'addition disomique et d'amphiploïdes partiels de Thinopyrum junceum sur le blé tendre. C R Acad Sci Paris Ser 3, 315:551–557
Chen Q, Jahier J, Cauderon Y (1989) Production and cytogenetical studies of hybrids between Triticum aestivum L. Thell. and Agropyron cristatum (L.) Gaertn. C R Acad Sci Paris Ser 3 308:411–416
Chen Q, Jahier J, Cauderon Y (1990) Intergeneric hybrids between Triticum aestivum and three crested wheatgrasses: Agropyron mongolicum, A. michnoi and A. desertorum. Genome 33:663–667
Chen Q, Jahier J, Cauderon Y (1992) Production and cytogenetic analysis of BC1, BC2, and BC3 progenies of an intergeneric hybrid between Triticum aestivum (L.) Thell. and tetraploid Agropyron cristatum (L.) Gaertn. Theor Appl Genet 84:698–703
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21
Devos KM, Atkinson MD, Chinoy CN, Francis HA, Harcourt RL, Koebner RMD, Liu CJ, Masojc P, Xie DX, Gale MD (1993) Chromosomal rearrangements in the rye genome relative to that of wheat. Theor Appl Genet 85:673–680
Dewey DR (1984) The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae. In: Gustafson JP (ed) Gene manipulation in plant improvement. 16th Stadler Genet Symposium. Plenum, New York, pp 209–279
Dvorak J, Knott DR (1974) Disomic and ditelosomic additions of diploid Agropyron elongatum chromosomes to Triticum aestivum. Can J Genet Cytol 16:399–417
Forster BP, Reader SM, Forsyth SA, Koebner RMD, Miller TE, Gale MD, Cauderon Y (1987) An assessment of the homoeology of six Agropyron intermedium chromosomes added to wheat. Genet Res 50:91–97
Gill BS, Morris KLD (1987) Assignment of the genomic affinities of chromosomes from polyploid Elymus species added to wheat. Genome 30:70–82
Hart GE, Tuleen NA (1983) Chromosomal locations of eleven Elytrigia elongata (=Agropyron elongatum) isozyme structural genes. Genet Res 41:181–202
Ishii T, Panaud O, Brar DS, Khush GS (1990) Use of non-radioactive, digoxigenin-labelled DNA probes for RFLP analysis in rice. Plant Mol Biol Rep 3:167–171
Jauhar PP (1992) Chromosome pairing in hybrids between hexaploid bread wheat and tetraploid crested wheatgrass (Agropyron cristatum). Hereditas 116:107–109
Jewell DC, Driscoll CJ (1983) The addition of Aegilops variabilis chromosomes to Triticum aestivum and their identification. Can J Genet Cytol 25:76–84
Li L, Dong Y (1991) Hybridization between Triticum aestivum L. and Agropyron michnoi Roshev. 1. Production and cytogenetic study of F1 hybrids. Theor Appl Genet 81:312–316
Limin AE, Fowler DB (1990) An interspecific hybrid and amphiploid produced from Triticum aestivum crosses with Agropyron cristatum and Agropyron desertorum. Genome 33:581–584
Lu YH, Merlino M, Isaac PG, Staccy J, Bernard M, Leroy P (1994) A comparative analysis between [32P] and digoxigenin-labelled single-copy probes for RFLP detection in wheat. Agronomie 14:33–39
Miller TE, Reader SM (1987) A guide to the homoeology of chromosomes within the Triticeae. Theor Appl Genet 74:214–217
Morris KLD, Raupp WJ, Gill BS (1989) Isolation of Ht-genome chromosome additions from polyploid Elymus trachycaulus (StStHtHt) into common wheat (Triticum aestivum). Genome 33:16–22
Panaud O, Magpantay G, McCouch S (1993) A protocol for non-radioactive DNA labelling and detection in the RFLP analysis of rice and tomato using single-copy probes. Plant Mol Biol Rep 11:54–59
Sharp PJ, Chao S, Desai S, Gale MD (1989) The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm. Theor Appl Genet 78:342–348
Xu YS, Jones MGK, Karp A, Pehu E (1993) Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes. Theor Appl Genet 85:1017–1022
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Communicated by K. Tsunewaki
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Chen, Q., Lu, Y.L., Jahier, J. et al. Identification of wheat-Agropyron cristatum monosomic addition lines by RFLP analysis using a set of assigned wheat DNA probes. Theoret. Appl. Genetics 89, 70–75 (1994). https://doi.org/10.1007/BF00226985
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DOI: https://doi.org/10.1007/BF00226985