Abstract
Restriction fragment analysis was used to examine the inheritance of lentil mitochondrial DNA (mtDNA) in F1 and F5 progeny from intrasubspecific (Lens culinaris ssp. culinaris) crosses and in F1 progeny from intersubspecific (Lens culinaris ssp. orientalis x L. culinaris ssp. culinaris) crosses. Southern blots of digested parental and progeny DNA were hybridized to heterologous maize mtDNA probes specific to coxI and atp6 genes. Two restriction fragment polymorphisms separated L.c. ssp. culinaris ‘Laird’ and ‘Eston’ from L.c. ssp. culinaris ‘ILL5588’, and one restriction fragment polymorphism distinguished L.c. ssp. culinaris ‘Laird’ and ‘Eston’ from L.c. ssp. orientalis ‘LO4’. Twelve of 13 f1 progeny and all F5 progeny from the intrasubspecific crosses, and all F1 progeny from intersubspecific crosses had only maternal mtDNA restriction fragments. One f1 plant from an ‘Eston’ x ‘ILL5588’ cross inherited mtDNA fragments from both parents. Nuclear DNA inheritance was biparental in all F1 progeny.
Similar content being viewed by others
References
Chomczynski P, Qasba PK (1984) Alkaline transfer of DNA to plastic membrane. Biochem Biophysic Res Commun 122:340–344
Dewey RE, Levings III CS, Timothy DH (1985) Nucleotide sequence of ATPase subunit 6 gene of maize mitochondria. Plant Physiol 79:914–919
Doyle DG, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Photochem Bull 19:11–15
Erickson L, Kemble R (1990) Paternal inheritance of mitochondria in rapeseed (Brassica napus). Mol Gen Genet 222:135–139
Havey MJ, Muehlbauer FJ (1989a) Variability for restriction fragment lengths and phylogenies in lentil. Theor Appl Genet 77:839–843
Havey MJ, Muehlbauer FJ (1989b) Linkage between restriction fragment length, isozyme and morphological markers in lentil. Theor Appl Genet 77:395–401
Horlow C, Goujaud J, Lepingle A, Missonier C, Bourgin J-P (1990) Transmission of paternal chloroplasts in tobacco (Nicotiana tabacum). Plant Cell Rep 9:249–252
Issac PG, Jones VP, Leaver CJ (1985) The maize cytochrome c oxidase subunit I gene: sequence, expression and rearrangement in cytoplasmic male sterile plants. EMBO J 4:1617–1623
Ladizinsky G, Braun D, Goshen D, Muehlbauer FJ (1984) The biological species of the genus Lens L. Bot Gaz 145:253–261
Maniatis T, Fritsch EF, Sambroke J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Monroy AF, Gao C, Zhang M, Brown GG (1990) Double-stranded RNA molecules in Brassica are inherited biparentally and appear not to be associated with mitochondria. Curr Genet 17:427–431
Muench DG, Slinkard AE, Scoles GJ (1991) Determination of genetic variation and taxonomy in lentil (Lens Miller) species by chloroplast DNA polymorphism. Euphytica 56:213–218
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) Chloroplast DNA evolution and origin of amphidiploid Brassica species. Theor Appl Genet 65:181–189
Radetzky R (1990) Analysis of mitochondrial DNA and its inheritance in Populus. Curr Genet 18:429–434
Rajora OP, Dancik BP (1992) Chloroplast DNA inheritance in Populus. Theor Appl Genet 84:280–285
Rajora OP, Barrett JW, Dancik BP, Strobeck C (1992) Maternal transmission of mitochondrial DNA in interspecific hybrids of Populus. Curr Genet 22:141–145
Schmitz UK (1988) Molecular analysis of mitochondrial DNA and its inheritance in Epibolium. Curr Genet 13:411–415
Schumann CM, Hancock JF (1989) Paternal inheritance of plastids in Medicago sativa. Theor Appl Genet 78:863–866
Simpson BB, Conner-Ogorzaly M (1986) Economic botany: plants in our world McGraw-Hill, New York
Smith SE (1989) Biparental inheritance of organelles and its implications in crop improvement. Plant Breed Rev 6:361–393
Soliman K, Fedak G, Allard RW (1987) Inheritance of organelle DNA in barley and Hordeum x Secale intergeneric hybrids. Genome 29:867–872
Vaillancourt RE, Slinkard AE (1992) Inheritance of new genetic markers in lentil (Lens Miller). Euphytica 64:227–236
Author information
Authors and Affiliations
Additional information
Communicated by H. F. Linskens
NRCC No. 38451
Rights and permissions
About this article
Cite this article
Rajora, O.P., Mahon, J.D. Inheritance of mitochondrial DNA in lentil (Lens culinaris Medik.). Theoret. Appl. Genetics 89, 206–210 (1994). https://doi.org/10.1007/BF00225143
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225143