Abstract
Rye chromosomes of wheat-rye addition lines were successfully identified by means of an RFLP analysis with 30 probes. Our results are in agreement with previous cytological data concerning the identity of lines F (+1R), D (+2R), C (+3R), A (+4R), E (+5R) and B (+7R). Two categories of chromosomal rearrangements have been distinguished, namely: (1) deletions: the current line D possesses a chromosome 2R deleted on its short arm and the line G a chromosome 3R deleted on its long arm; we have also noticed a deletion on the long arm of wheat chromosome 1A in line F61; and (2) evolutionary reciprocal translocations in rye relative to wheat which have been previously mentioned in the literature. The anther culture response of the different lines was studied. A significant difference between ‘FEC 28’ and the addition lines was observed for embryo production and plant regeneration. It appears that genes located on ‘S 10’ chromosome arm 3RL and on ‘FEC 28’ chromosome arm 1AL increase embryo frequency whereas gene(s) located on ‘S 10’ chromosome 5R reduce(s) it. Plant regeneration results suggest that genes increasing regeneration ability and green-plant frequency are located on ‘S 10’ chromosome 4R. The long arm of chromosome 1A seems to be involved positively in green-plant regeneration whereas chromosomes 1R and 3R limit plant regeneration.
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References
Agache S, De Buyser J, Henry Y, Snape J (1988) Studies of the genetic relationship between anther culture and somatic tissue culture abilities in wheat. Plant Breed 100:26–33
Agache S, Bachelier B, De Buyser J, Henry Y, Snape J (1989) Genetic analysis of anther culture response in wheat using aneuploid, chromosome substitution and translocation lines. Theor Appl Genet 77:7–11
Ainsworth CC, Miller TE, Gale MD (1986) The genetic control of grain esterases in hexaploid wheat. 2. Homoeologous loci in related species. Theor Appl Genet 72:219–225
Andersen SB, Due IK, Olesen A (1987) The response of anther culture in genetically wide material of winter wheat (Triticum aestivum L.). Plant Breed 99:181–186
Bernard M (1976) Etude des caractéristiques cytologiques, morphologiques et agronomiques de six lignées d'addition blé-seigle. Ann Amélior Plant 26:67–91
Bernard S (1977) Etude de quelques facteurs contribuant à la réussite de l'androgénèse par culture d'anthères in vitro chez le Triticale hexaploide. Ann Amélior Plant 27:639–655
Bernard S (1989) Direct embryogenesis and plant production through in vitro androgenesis in Triticale and wheat (abstract). In: Science for plant breeding, XIIth EUCARPIA Congress, Göttingen (RFA)
Bernard S, Faurie C, Madéore A, Martinez I, Bernard M (1993) Progress in wheat and Triticale anther culture through identification of environmental and genetic factors. Utilisation of doubled haploids in genetics and breeding. In: VIIIth Int Wheat Genet Symp, Beïjing (China) (in press)
Chao S, Sharp PJ, Worland AJ, Warham EJ, Koebner RMD, Gale MD (1989) RFLP-based genetic maps of wheat homoeologous group-7 chromosomes. Theor Appl Genet 78:495–504
Charmet G, Bernard S (1984) Diallel analysis of androgenetic plant production in hexaploid Triticale (X Triticosecale, Wittmack). Theor Appl Genet 69:55–61
Chuang CC, Ouyang TW, Chia H, Chou SM, Chin CK (1978) A set of potato media for wheat anther culture. In: Proc Symp Plant Tissue Culture, Science Press Peking, pp 51–56
Daniel G (1993) Anther culture in rye: improved plant regeneration using modified MS media. Plant Breed 110:259–261
Darvey NL, Gustafson JP (1975) Identification of rye chromosomes in wheat-rye addition lines and Triticale by heterochromatin bands. Crop Sci 15:239–243
Deaton WR, Metz SG, Armstrong TA, Mascia PN (1987) Genetic analysis of the anther-culture response of three spring wheat crosses. Theor Appl Genet 74:334–338
De Buyser J, Henry Y, Lonnet P, Hertzog R, Hespel A (1987) ‘Florin’: a doubled haploid wheat variety developed by the anther culture method. Plant Breed 98:53–56
De Buyser J, Hachemi-Rachedi S, Lemee ML, Sejourne S, Marcotte JL, Henry Y (1992) Aneuploid analysis of anther culture response in wheat. Plant Breed 109:339–342
Devos KM, Atkinson MD, Chinoy CN, Francis HA, Harcourt RL, Koebner RMD, Liu CJ, Masojc P, Xie DX, Gale MD (1993 a) Chromosomal rearrangements in the rye genome relative to that of wheat. Theor Appl Genet 85:673–680
Devos KM, Millan T, Gale MD (1993 b) Comparative RFLP maps of the homoeologous group-2 chromosomes of wheat, rye and barley. Theor Appl Genet 85:784–792
Devos KM, Gale M (1993 c) The genetic maps of wheat and their potential in plant breeding. Outlook Agric 22:93–99
Foroughi-Wehr B, Zeller FJ (1990) In vitro microspore reaction of different German wheat cultivars. Theor Appl Genet 79:77–80
Foroughi-Wehr B, Friedt W, Wenzel G (1982) On the genetic improvement of androgenetic haploid formation in Hordeum vulgare L. Theor Appl Genet 62:233–239
Henry Y, De Buyser J (1985) Effect of the 1B/1R translocation on anther-culture ability in wheat (Triticum aestivum L.). Plant Cell Rep 4:307–310
Henry Y, De Buyser J (1990) Wheat anther culture: agronomic performance of doubled haploid lines and the release of a new variety ‘Florin’. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Vol 13, wheat. Springer-Verlag, Berlin, pp 286–352
Henry Y, Bernard S, Bernard M, Gay G, Marcotte JL, De Buyser J (1993) Nuclear gametophytic genes from chromosome arm 1RS improve regeneration of wheat microspore-derived embryos. Genome 36:808–814
Hu DF, Yuan ZD, Tang YL, Liu JP (1986) Jinghua no. 1, a winter wheat variety derived from pollen sporophyte. Sci Sin 28:733–745
Jouve N, Diaz F (1990) Genetic control of esterase-6 isozymes in hexaploid wheat and rye. Euphytica 47:165–169
Jouve N, Galindo C, Mesta M, Diaz F, Albella B, Garcia P, Soller C (1989) Changes in Triticale chromosome heterochromatin visualized by C-banding. Genome 32:735–742
Lashermes P, Engin G, Ortiz-Ferrara G (1991) Anther culture of wheat (Triticum aestivum) adapted to dry areas of West Asia and North Africa. J Genet Breed 45:33–38
Lazar MD, Baenziger PS, Schaeffer GW (1984) Combining abilities and heritability of callus formation and plantlet regeneration in wheat (Triticum aestivum L.) anther culture. Theor Appl Genet 68:131–134
Lazar MD, Chen THH, Scoles GJ, Kartha KK (1987) Immature embryo and anther culture of chromosome addition lines of rye in Chinese Spring wheat. Plant Sci 51:77–81
Law CN (1981) Chromosome manipulation in wheat. Chromosomes Today 7:194–205
Liu CL, Atkinson MD, Chinoy CN, Devos KM, Gale MD (1992) Nonhomoeologous translocations between group-4, -5 and -7 chromosomes within wheat and rye. Theor Appl Genet 83:305–312
Miller TE (1984) The homoeologous relationship between the chromosomes of rye and wheat. Current status. Can J Genet Cytol 26:578–589
Miller TE, Reader SM (1987) A guide to the homoeology of chromosomes within the Triticeae. Theor Appl Genet 74:214–217
Naranjo T, Fernandez-Rueda P (1991) Homoeology of rye chromosome arms to wheat. Theor Appl Genet 82:577–586
Orlov PA, Mavrishcheva EB, Palilova AN (1993) Estimation of the response to anther culturing in 60 genotypes of different wheat species. Plant Breed 111:339–342
Quimio CA, Zapata FJ (1990) Diallel analysis of callus induction and green-plant regeneration in rice anther culture. Crop Sci 30:188–192
Riley R, Chapman V (1958) The production and phenotypes of wheat-rye chromosome addition lines. Heredity 12:301–305
Rogers SO, Bendich AJ (1985) Extraction of DNA from milligram amounts of fresh herbarium and mummified plant tissues. Plant Mol Biol 5:69–76
Rognli OA, Devos KM, Chinoy CN, Harcourt RL, Atkinson MD, Gale MD (1992) RFLP mapping of rye chromosome 7R reveals a highly translocated chromosome relative to wheat. Genome 35:1026–1031
Sharp PJ, Kreis M, Shewry PR, Gale MD (1988) Location of β-amylase sequences in wheat and its relatives. Theor Appl Genet 75:286–290
Sharp PJ, Chao S, Desai S, Gale MD (1989) The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm. Theor Appl Genet 78:342–348
Sybenga J (1983) Rye chromosome nomenclature and homoeology relationships, workshop report. Z Pflanzenzücht 90:297–304
Szakacs E, Kovacs G, Pauk J, Barnabas B (1988) Substitution analysis of callus induction and plant regeneration from anther culture in wheat (Triticum aestivum L.). Plant Cell Rep 7:127–129
Tuvesson IKD, Pedersen S, Andersen SB (1989) Nuclear genes affecting albinism in wheat (Triticum aestivum L.) anther culture. Theor Appl Genet 78:879–883
Wang ML, Atkinson MD, Chinoy CN, Devos KM, Harcourt RL, Liu CJ, Rogers WJ, Gale MD (1991) RFLP-based genetic map of rye (Secale cereale L.) chromosome 1R. Theor Appl Genet 82:174–178
Wenzel G, Thomas E (1974) Observations on the growth in culture of anthers of Secale cereale. Z Pflanzenzücht 72:89–94
Zhang YL, Li DS (1984) Anther culture of monosomics in Triticum Aestivum. Hereditas (Beijing) 6:7–10
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Martinez, I., Bernard, M., Nicolas, P. et al. Study of androgenetic performance and molecular characterisation of a set of wheat-rye addition lines. Theoret. Appl. Genetics 89, 982–990 (1994). https://doi.org/10.1007/BF00224528
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DOI: https://doi.org/10.1007/BF00224528