Abstract
Sugar beet (Beta vulgaris L.) is highly susceptible to the beet cyst nematode (Heterodera schachtii Schm.). Three resistance genes originating from the wild beets B. procumbens (Hs1 pro-1) and B. webbiana (Hs1 web-1, Hs2 web-7) have been transferred to sugar beet via species hybridization. We describe the genetic localization of the nematode resistance genes in four different sugar beet lines using segregating F2 populations and RFLP markers from our current sugar beet linkage map. The mapping studies yielded a surprising result. Although the four parental lines carrying the wild beet translocations were not related to each other, the four genes mapped to the same locus in sugar beet independent of the original translocation event. Close linkage (0–4.6 cM) was found with marker loci at one end of linkage group IV. In two populations, RFLP loci showed segregation distortion due to gametic selection. For the first time, the non-randomness of the translocation process promoting gene transfer from the wild beet to the sugar beet is demonstrated. The data suggest that the resistance genes were incorporated into the sugar beet chromosomes by non-allelic homologous recombination. The finding that the different resistance genes are allelic will have major implications on future attempts to breed sugar beet combining the different resistance genes.
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References
Barzen E, Mechelke W, Ritter E, Schulte-Kappert E, Salamini F (1995) An extended map of the sugar beet genome containing RFLP and RAPD loci. Theor Appl Genet 90:189–193
Boudry P, Wieber R, Saumitou-Laprade P, Pillen K, Van Dijk H, Jung C (1994) Identification of RFLP markers closely linked to the bolting gene B and their significance for the study of the annual habit in beets (Beta vulgaris L.). Theor Appl Genet 88:852–858
Ceoloni C, Del Signore G, Ercoli L, Donini P (1992) Locating the alien chromatin segment in common wheat-Aegilops longissima mildew resistant transfers. Hereditas 116:239–245
De Jong JH, Speckmann GJ, De Bock TSM, Van Voorst A (1985) Monosomic additions with resistance to beet cyst nematode obtained from hybrids of Beta vulgaris and wild Beta species of the section Patellares. II. Comparative analysis of the alien chromosomes. Z Pflanzenzuecht 95:84–94
Haldane JBS (1919) The combination of linkage values and the calculation of distance between the loci of linked factors. J Genet 8:299–309
Hart CM, Fischer B, Neuhaus JM, Meins F (1992) Regulated inactivation of homologous gene expression in transgenic Nicotiana sylvestris plants containing a defense-related tobacco chitinase gene. Mol Gen Genet 235:179–188
Heijbroek W, Roelands AJ, de Jong JH, van Hulst C, Schoone AHL, Munning RG (1988) Sugar beets homozygous for resistance to beet cyst nematode (Heterodera schachtii Schm.) developed from monosomic additions of Beta procumbens to B. vulgaris. Euphytica 38:121–131
Jung C, Herrmann RG (1991) A DNA probe for rapid screening of sugar beet (Beta vulgaris L.) carrying extra chromosomes from wild beets of the Procumbentes section. Plant Breed 107:275–279
Jung C, Wricke G (1987) Selection of diploid nematode-resistant sugar beet from monosomic addition lines. Plant Breed 98:205–214
Jung C, Koch R, Fischer F, Brandes A, Wricke G, Herrmann RG (1992) DNA markers closely linked to nematode resistance genes in sugar beet (Beta vulgaris L.) using chromosome additions and translocations originating from wild beets of the Procumbentes species. Mol Gen Genet 232:271–278
Jung C, Pillen K, Frese L, Fähr S, Melchinger AE (1993) Phylogenetic relationships between cultivated and wild species of the genus Beta revealed by DNA “fingerprinting”. Theor Appl Genet 86:449–457
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Lange W, Müller J, De Bock TSM (1993) Virulence in the beet cyst nematode (Heterodera schachtii) versus some alien genes for resistance in beet, Fundam Appl Nematol 16:447–454
Löptien H (1984a) Breeding nematode-resistant beets. I. Development of resistant alien additions by crosses between Beta vulgaris L. and wild species of the section Patellares. Z Pflanzenzuecht 92:208–220
Löptien H (1984b) Breeding nematode-resistant beets. II. Investigations into the inheritance of resistance to Heterodera schachtii Schm. in wild species of the section Patellares. Z Pflanzenzuecht 93:237–245
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in species genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Pillen K, Steinrücken G, Hermann RG, Jung C (1993) An extended linkage map of sugar beet (Beta vulgaris L.) including nine putative lethal genes and the restorer geneX. Plant Breed 111:265–272
Quin M, Bayley C, Stockton T, Ow DW (1994) Cre recombinase-mediated site-specific recombination between plant chromosomes. Proc Natl Acad Sci USA 91:1706–1710
Reamon-Ramos SM, Wricke G (1992) A full set of monosomic addition lines in Beta vulgaris from Beta webbiana: morphology and isozyme markers. Theor Appl Genet 84:411–418
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Salentijn EMJ, Sandal NN, Lange W, De Bock TSM, Krens FA, Marcker KA, Stiekema WJ (1992) Isolation of DNA markers linked to a beet cyst nematode resistance locus in Beta patellaris and Beta procumbens. Mol Gen Genet 235:432–440
Savitsky H (1975) Hybridization between Beta vulgaris and B. procumbens and transmission of nematode (Heterodera schachtii) resistance to sugarbeet. Can J Genet Cytol 17:197–209
Schmidt T, Jung C, Metzlaff M (1991) Distribution and evolution of two satellite DNAs in the genus Beta. Theor Appl Genet 82:793–799
Speckmann GJ, De Bock TSM, de Jong JH (1985) Monosomic additions with resistance to beet cyst nematode obtained from hybrids of Beta vulgaris and wild Beta species of the section Patellares. I. Morphology transmission and level of resistance. Z Pflanzenzuecht 95:74–83
Toxopeus JH, Lubberts H (1979) Breeding for resistance to the sugar beet nematode (Heterodera schachtii Schm.) in cruciferous crops. In: Post Conference Edition Eucarpia Crucif Conf. PUDOC, Wageningen, pp 151
Uphoff H, Wricke G (1992) Random amplified polymorphic DNA (RAPD) markers in sugar beet (Beta vulgaris L.): mapping the genes for nematode resistance and hypocotyl colour. Plant Breed 109:168–171
Wagner H, Gimbel EM, Wricke G (1989) Are Beta procumbens Chr. Sm. and Beta webbiana Moq. different species? Plant Breed 102:17–21
Wagner H, Weber WE, Wricke G (1992) Estimating linkage relationship of isozyme markers and morphological markers in sugar beet (Beta vulgaris L.) including families with distorted segregations. Plant Breed 108:89–96
Yu MH (1981) Sugar beets homozygous for nematode resistance and transmission of resistance to their progeny. Crop Sci 21:714–717
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Communicated by G. Wenzel
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Heller, R., Schondelmaier, J., Steinrücken, G. et al. Genetic localization of four genes for nematode (Heterodera schachtii Schm.) resistance in sugar beet (Beta vulgaris L.). Theoret. Appl. Genetics 92, 991–997 (1996). https://doi.org/10.1007/BF00224039
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DOI: https://doi.org/10.1007/BF00224039