Summary
This study measured the velocity of fast orthograde axonal transport of incorporated 3H-proline in motoneurones of the sciatic nerve in control rats and in rats with streptozotocin-induced diabetes of 3 weeks duration. Sciatic nerve and abdominal cavity temperatures were monitored throughout the period of measurement of transport velocity, and the rats were warmed to minimise hypothermia at both sites. There was marked abdominal and sciatic nerve hypothermia immediately after operation, and this effect was more intense in diabetic rats than in control rats. In steady state, abdominal cavity temperature (mean±SEM) was 38.1±0.1 °C in both control and diabetic rats, and the sciatic nerve temperatures were 37.8±0.1 °C in controls and 37.1±0.3 °C in diabetic rats. The difference was not statistically significant. The velocities of orthograde axonal transport for the fastest molecules containing 3H-proline were 14.0±0.9 (SEM)mm/h for controls and 13.9±1.1 (SEM)mm/h for diabetic rats. Thus, no velocity difference was observed. The findings are discussed in relation to measurements of fast orthograde transport velocity in experimental diabetes in other studies. It is suggested that, where velocity deficits have been seen in diabetic rats, nerve hypothermia should be considered as a contributory factor.
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Perísíc M, Cuénod M (1972) Synaptic transmission depressed by colchicine blockade of axoplasmic flow. Science 175: 1140–1142
Sidenius P, Jakobsen J (1979) Axonal transport in early experimental diabetes. Brain Res 173: 315–330
Bisby MA (1980) Axonal transport of labelled protein and regeneration rate in nerves of streptozotocin diabetic rats. Exp Neurol 69: 74–84
Bajada S, Sharma AK, Thomas PK (1980) Axoplasmic transport in vagal afferent fibres in normal and alloxan-diabetic rabbits. J Neurol Sci 47: 365–378
Meiri KF, McLean WG (1982) Axonal transport of protein in motor fibres of experimentally diabetic rats — fast anterograde transport. Brain Res 238: 77–88
Mendell JR, Sahenk Z, Warmolts JR, Marshall JK, Thibert P (1982) The spontaneously diabetic BB Wistar rat. Morphologic and physiologic studies of peripheral nerve. J Neurol Sci 52: 103–115
Bisby MA, Jones DL (1978) Temperature sensitivity of axonal transport in hibernating and non-hibernating rodents. Exp Neurol 61: 74–83
Brimijoin S, Olsen J, Rosenson R (1979) Comparison of the temperature-dependence of rapid axonal transport and microtubules in nerves of the rabbit and bullfrog. J Physiol 287: 303–314
Brown AM, Slater EJ, Tomlinson DR (1985) Analysis of axonal transport of radiolabelled tracer molecules using a microcomputer. J Physiol 361: 10P
Sokal RR, Rohlf FJ (1981) Biometry, 2nd edn. Freeman, New York, pp 226–227
Bisby MA (1976) Orthograde and retrograde axonal transport of labelled protein in motoneurones. Exp Neurol 50: 628–640
Ochs S (1972) Rate of fast axoplasmic transport in mammalian nerve fibres. J Physiol 227: 627–645
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Whiteley, S.J., Townsend, J., Tomlinson, D.R. et al. Fast orthograde axonal transport in sciatic motoneurones and nerve temperature in streptozotocin-diabetic rats. Diabetologia 28, 847–851 (1985). https://doi.org/10.1007/BF00291076
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DOI: https://doi.org/10.1007/BF00291076