Summary
The insulin-like activity (ILA) of rat serum and of insulin-adrenaline mixtures was estimated by both rat diaphragm and rat adipose tissue methods and expressed in μ units/ml. The ILA of the rat serum strikingly increases after demedullation or transplantation of the adrenal gland; the addition of adrenaline to normal rat serum decreases its ILA. In “in vitro” experiments, it was found that adrenaline exhibits an inhibitory but not antagonistic effect upon insulin activity, i.e. that adrenaline has no action upon muscle or adipose tissue in the absence of insulin. Finally the rise of the ILA provoked by dilution of rat serum seems to be due to the dilution of inhibitory substances (such as adrenaline) and to the liberation of an insulin fraction which is masked in the whole serum.
Résumé
L'≪insulinémie apparente≫ a été estimée dans des serums de rats ou des mélanges d'insuline et d'adrénaline par les méthodes du diaphragme et du tissu adipeux de rat. Chez le rat, l'autogreffe ou l'énucléation de la surrénale augmentent l'≪insulinémie apparente≫, alors que l'adjonction d'adrénaline à un sérum normal la diminue. L'adrénaline, à concentrations physiologiques, n'agit pas en antagoniste, mais en inhibiteur de l'insuline. Enfin, l'augmentation de l'≪insulinémie apparente≫ consécutive à la dilution du sérum semble être due, à la fois, à la dilution des substances antagonistes et inhibitrices, en particulier de l'adrénaline, et à la libération d'une fraction insulinique normalement masquée dans le sérum entier.
Zusammenfassung
Die insulinähnliche Aktivität von Rattenserum und Insulin-Adrenalin-Mischungen wurde mit der Zwerchfell- sowie mit der Fettgewebs-Methode gemessen und in μE/ml ausgedrückt. Demedullation oder Transplantation der Nebenniere bei der Ratte steigert die insulinähnliche Aktivität, die Zugabe von Adrenalin zum Normalserum dagegen vermindert sie. In physiologischen Konzentrationen wirkt Adrenalin nicht als Antagonist, sondern als Inhibitor des Insulins, da Adrenalin in Abwesenheit des Insulins ohne Effekt auf das Zwerchfell und auf das Fettgewebe ist. Die durch Verdünnen des Serums auftretende Steigerung der insulinähnlichen Aktivität scheint zwei Ursachen zu haben: Die Verdännung der hemmenden Substanzen (Adrenalin) und die Freisetzung einer im unverdünnten Serum unwirksamen Insulinfraktion.
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Mialhe, P., Sutter, B.C.J., Meyer, V. et al. L'adrénaline, inhibiteur de l'insuline sérique chez le rat. Diabetologia 1, 63–71 (1965). https://doi.org/10.1007/BF01338717
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DOI: https://doi.org/10.1007/BF01338717