Summary
Immunoelectrophoresis of glomerular basement membrane antigens in the urine of 20 Type 1 (insulin-dependent) diabetic and 10 healthy children was performed. In 10 of the diabetic children, there was altered α-1-mobility, while the other diabetic and normal children showed α-2-mobility. After incubation with glucose, glomerular basement membrane antigens in the urine of healthy children showed α-1-mobility. Isolated human kidney glomerular basement membrane split products obtained by proteolytic degradation (papain, trypsin, chymotrypsin) were also investigated by immunoelectrophoresis. A difference was observed in the immunoelectrophoretic pattern of native and glycosylated glomerular basement membrane split products. A distinct increase of thiobarbituric acid assay positive glomerular basement membrane structures after incubation with glucose provides suggestive evidence for the occurrence of non-enzymatic glycosylation of glomerular basement membrane proteins. Glycosylated glomerular basement membrane proteins may contribute to both functional and morphological changes in diabetic glomerulosclerosis.
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Schober, E., Pollak, A., Coradello, H. et al. Glycosylation of glomerular basement membrane in type 1 (insulin-dependent) diabetic children. Diabetologia 23, 485–487 (1982). https://doi.org/10.1007/BF00254295
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DOI: https://doi.org/10.1007/BF00254295