Summary
Antigens on the rat pancreatic islet cell surface were redistributed into patch and cap formation when the cells were incubated in the presence of the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine, in tissue culture medium 199 for 24 h, before addition of rat pancreatic islet cell surface antibody. In contrast, if the cells were cultured in tissue culture medium 199 supplemented with glucose (5.5 or 16.7mmol/l) and 10% heat-inactivated fetal calf serum without 3-isobutyl-1-methylxanthine, cap formation was not detectable. These results suggest that mobile antigen on the surface of pancreatic B cells can be induced to aggregate into patch and cap formations during conditions of increased cellular metabolism.
Article PDF
Similar content being viewed by others
References
Bottazo GF, Florin-Christensen A, Doniach D (1974) Islet cell antibodies in diabetes mellitus with autoimmuno polyendocrine deficiencies. Lancet 2: 1279–1283
MacCuish AC, Jordan J, Campbell CJ, Duncan LJP, Irvine WJ (1974) Antibodies to islet-cells in insulin-dependent diabetics with coexistent autoimmune disease. Lancet 2: 1529–1533
Lernmark Å, Freedman ZR, Hoffman C, Rubenstein AH, Steiner DF, Jackson RL, Winter RJ, Traisman HS (1978) Islet cell surface antibodies in juvenile diabetes mellitus. N Engl J Med 299: 375–380
Irvine WJ, Gray RS, McCallum CJ, Duncan LJP (1977) Clinical and pathogenic significance of pancreatic islet cell antibodies in diabetics treated with oral hypoglycaemic agents. Lancet 1: 1025–1027
Lernmark Å, Winbland B (1977) Scanning electron microscopy of surface change on dispersed pancreatic cells following stimulation of insulin release. Med Biol 55: 141–147
Lernmark Å, Nathana A, Steiner DF (1976) Preparation and characterization of plasma membrane-enriched fractions from rat pancreatic islet. J Cell Biol 71: 606–623
Ohgawara H, Carroll R, Hoffmann C, Takahashi C, Kikuchi M, Labrecque A, Hirata Y, Steiner DF (1978) Promotion of monolayer formation in cultured whole pancreatic islets by 3-isobutyl-1-methylxanthine. Proc Natl Acad Sci 75: 1897–1900
Sakurai Y, Akaike T, Kataoka K, Okano T (1980) Interfacial phenomena in biomaterial chemistry. In: Goldberg E, Nakajima A (eds) Biomedical polymers. Academic Press, New York, pp 335–379
Lernmark Å, Kanastuna T, Patzelt C, Diakoumis K, Carroll R, Rubenstein AH, Steiner DF (1980) Antibodies directed against the pancreatic islet cell plasma membrane. Diabetologia 19: 445–451
Sanders SK, Alexander EL, Braylan RC (1975) A high-yield technique for preparing cells fixed in suspension for scanning electron microscopy. J Cell Biol 67: 476–480
Tayer RB, Duffus WHPH, Raff MC, De Petris S (1971) Redistribution and phagocytosis of lymphocyte surface immunglobulin molecules induced by anti-immunoglobulin antibody. Nature New Biol 233: 225–230
De Petris S (1978) Distribution and mobility of plasma membrane components on lymphocytes in dynamic aspect of cell surface organization. In: Poste G, Nicolsen GL (eds) Cell surface review, vol. 3. North Holland, Amsterdam, pp 643–728
Schreiner GF, Unanue ER (1976) Membrane and cytoplasmic changes in B lymphocytes induced by ligand-surface immunoglobulin interaction. Adv Immunol 24: 34–165
Yahara I, Kakimoto-Sameshima F (1977) Ligand-independent cap formation: Redistribution of surface receptors on mouse lymphocytes and thymocytes in hypertonic medium. Proc. Natl Acad Sci 74: 5411–4515
Schreiner GF, Braun J, Unanue ER (1976) Spontaneous redistribution of surface immunoglobulin in the motile B lymphocyte. J Exp Med 144: 1683–1688
De Petris S, Rafi MC (1972) Distribution of immunglobulin on the surface of mouse lymphoid cells as determined by immunoferritin electron microscopy: Antibody-induced, temperature-dependent redistribution and its implications for membrane structure. Eur J Immunol 2: 523–535
Loor F, Forni L, Pernis B (1972) The dynamic state of the lymphocyte membrane: factors affecting the distribution and turnover of surface immunglobulins. Eur J Immunol 2: 203–212
Gabbiani G, Chapnnier C, Zumbe A, Vassalli P (1977) Actin and tubulin co-cap with surface immunoglobulins in mouse lymphocytes. Nature 269: 687–698
Sundvist KG, Ehrnst A (1976) Cytoskeletal control of surface membrane mobility. Nature 264: 226–231
Puck TT, Waldren CA, Hsie AW (1972) Membrane dynamics in the action of dibutyl adenosine 3′:5-cyclic monophosphate and testosterone on mammalians cells. Proc Natl Acad Sci 69: 1943–1947
Puck TT (1977) Cyclic AMP, the microtubulus-microfilament system, and cancer. Proc Natl Acad Sci 74: 4491–4495
De Petris S (1978) Preferential distribution of surface immunoglobulins on microvilli. Nature 272: 66–68
Loor F, Hagg, LB (1975) The modulation of microprojections on the lymphocyte membrane and the redistribution of membrane-bound ligands, a correlation. Eur J Immunol 5: 854–865
Yahara I, Kakimoto-Sameshima F (1979) Mechanism of translocation of microvilli accompanying cap formation of surface receptors. Cell Structure and Function. 4: 143–152
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ohgawara, H., Okano, T., Kataoka, K. et al. Distribution and redistribution of pancreatic islet cell surface antigen reactive with islet cell surface antibody in the rat. Diabetologia 24, 117–121 (1983). https://doi.org/10.1007/BF00297393
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00297393