Summary
The activities and expression of protein kinase C isoenzymes were examined in glomerular mesangial cells cultured under high glucose conditions. Exposure of cells to high glucose concentrations (27.8 mmol/l) for more than 3 days resulted in a significant elevation of protein kinase C activities in the membrane fraction. Of the protein kinase C isoenzymes, the levels of protein kinase C α significantly increased in the membrane fraction after 3 days of exposure to glucose, and protein kinase C ζ increased after 5 days of exposure. Levels of protein kinase C δ and ε remained unchanged and protein kinase C Β and γ were not detected. These results indicate that protein kinase C α and ζ are translocated under high glucose conditions possibly through different mechanisms.
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Abbreviations
- PKC:
-
Protein kinase C
- DAG:
-
diacylglycerol
- PMSF:
-
phenylmethylsulfonyl fluoride
- TPA:
-
12-O-tetradecanoylphorbol 13-acetate
- DEAE cellulose:
-
diethylaminoethyl cellulose
- kDa:
-
kilodalton
References
Nishizuka Y (1992) Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C. Science 258: 607–614
Craven PA, DeRubertis FR (1989) Protein kinase C is activated in glomeruli from streptozotocin diabetic rats: possible mediation by glucose. J Clin Invest 83: 1667–1675
Ayo SH, Radnik R, Garoni JA, Troyer DA, Kreisberg JI (1991) High glucose increases diacylglycerol mass and activates protein kinase C in mesangial cell culture. Am J Physiol 261: F571-F577
Kikkawa R, Kitamura E, Fujiwara Y, Arimura T, Haneda M, Shigeta Y (1986) Impaired contractile responsiveness of diabetic glomeruli to angiotensin II: a possible indication of mesangial dysfunction in diabetes mellitus. Biochem Biophys Res Commun 136: 1185–1190
Kikkawa R, Haneda M, Togawa M, Koya D, Kajiwara N, Shigeta Y (1993) Differential modulation of mitogenic and metabolic actions of insulin-like growth factor I in rat glomerular mesangial cells in high glucose culture. Diabetologia 36: 276–281
Haneda M, Kikkawa R, Koya D, Uzu T, Maeda S, Togawa M, Shigeta Y (1993) Alteration of mesangial response to ANP and angiotensin II by glucose. Kidney Int 44: 518–526
Huwiler A, Fabbro D, Pfeilschifter J (1991) Possible regulatory functions of protein kinase C-α and -ε isoenzymes in rat renal mesangial cells: stimulation of prostaglandin synthesis and feedback inhibition of angiotensin II-stimulated phosphoinositide hydrolysis. Biochem J 279: 441–445
Huwiler A, Fabbro D, Stabel S, Pfeilschifter J (1992) Immunocharacterization of δ-and ζ-isoenzymes of protein kinase C in rat renal mesangial cells. FEBS Lett 300: 259–262
Saxena R, Saksa BA, Fields AP, Ganz MB (1993) Activation of Na/H exchanger in mesangial cells is associated with translocation of PKC isoforms. Am J Physiol 265: F53-F60
Nakanishi H, Brewer KA, Exton JH (1993) Activation of the ζ isoenzymes of protein kinase C by phosphatidylinositol 3,4,5-trisphosphate. J Biol Chem 268: 13–16
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Kikkawa, R., Haneda, M., Uzu, T. et al. Translocation of protein kinase C α and ζ in rat glomerular mesangial cells cultured under high glucose conditions. Diabetologia 37, 838–841 (1994). https://doi.org/10.1007/BF00404342
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DOI: https://doi.org/10.1007/BF00404342