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Synthesis of β-glucanase and other extracellular proteins by cells and protoplasts of the yeast Pichia polymorpha

Effect of 2-deoxy-d-glucose

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Abstract

The synthesis of β-glucanase either by cells or by protoplasts of the yeast Pichia polymorpha has been found to occur in the presence of 2-deoxy-d-glucose in the growth medium. On the other hand, the synthesis of typical extracellular proteins such as invertase and acid phosphatase is strongly affected by the presence of the drug. The degree of inhibition is, however, directly related to the 2-deoxy-d-glucose concentration.

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References

  • Farkas, V., Svoboda, A., Bauer, S.: Inhibitory effect of 2-deoxy-d-glucose on the formation of cell wall in yeast protoplasts. J. Bact. 98, 744–748 (1969)

    Google Scholar 

  • Heredia, C. F., de la Fuente, G., Sols, A.: Metabolic studies with 2-deoxyhexose. I. Mechanisms of inhibition of growth and fermentation in baker's yeast. Biochim. biophys. Acta (Amst.) 86, 216–223 (1964)

    Google Scholar 

  • Huggett, A. St. G., Nixon, D. A.: Use of glucose oxidase, peroxidase and o-dianisidine in determination of blood and urinary glucose. Lancet 1957II, 368

  • Johnson, B. F.: Lysis of yeast cell walls induced by 2-deoxy-glucose at their sites of glucan synthesis. J. Bact. 95, 1169–1172 (1968)

    Google Scholar 

  • Villa, T. G., Notario, V., Villanueva, J. R.: β-Glucanases of the yeast Pichia polymorpha. Arch. Microbiol. 104, 201–206 (1975)

    Google Scholar 

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Villa, T.G., Notario, V., Benítez, T. et al. Synthesis of β-glucanase and other extracellular proteins by cells and protoplasts of the yeast Pichia polymorpha . Arch. Microbiol. 105, 335–337 (1975). https://doi.org/10.1007/BF00447154

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  • DOI: https://doi.org/10.1007/BF00447154

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