Abstract
For lindane degradation, a cell suspension of Clostridium rectum strain S-17 demands the addition of substrates such as leucine, alanine, pyruvate, a leucine-proline mixture, and molecular hydrogen. In the presence of leucine-proline mixture, lindane decomposed in parallel with isovaleric acid formation, and both lindane degradation and isovaleric acid formation were inhibited by monoiodoacetic acid, suggesting a close relation between lindane degradation and the Stickland reaction. Lindane was degraded by cell-free extracts of C. rectum in the presence of dithiothreitol (DTT). Radiogaschromatograms of n-hexane soluble metabolites from [14C] lindane showed the presence of monochlorobenzene and γ-3,4,5,6-tetrachlorocyclohexene, Leucine, NADH, and NADPH were somewhat less active than DTT for lindane degradation in cell-free extracts. Reductive dechlorination seemed the major route of lindane degradation in cell-free extracts as well as in the intact cells of C. rectum.
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Abbreviations
- Lindane (γ-HCH):
-
γ-1,2,3,4,5,6-hexachlorocyclohexane
- α-HCH:
-
α-1,2,3,4,5,6-hexachlorocyclohexane
- γ-TCH:
-
γ-3,4,5,6-tetrachlorocyclohexene
- γ-PCH:
-
γ-1,3,4,5,6-pentachlorocyclohexene
- DTT:
-
1,4-dithiothreitol
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Ohisa, N., Yamaguchi, M. & Kurihara, N. Lindane degradation by cell-free extracts of Clostridium rectum . Arch. Microbiol. 125, 221–225 (1980). https://doi.org/10.1007/BF00446880
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DOI: https://doi.org/10.1007/BF00446880