Abstract
A simple rapid method for preparing synchronous cultures of Bacillus subtilis has been used to investigate changes in density during the cell cycle. Asynchronous cells separated on a stepped Percoll density gradient had a mean cell density of 1.117 g ml-1±0.004. Samples from a synchronous culture exhibited variation (ca. 1.5%) in mean cell density which was greatest at the onset of cell division. An asynchronous control culture showed little variation in density. These results are discussed in relation to previous work on Escherichia coli.
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References
Carty CE, Ingram LO (1981) Lipid synthesis during the Escherichia coli cell cycle. J Bacteriol 145:472–478
Chagla AH, Griffiths AJ (1978) Synchronous cultures of Acanthamoeba castellanii and their use in the study of encystation. J Gen Microbiol 108:39–43
Edwards C, Jones CW (1977) Respiratory properties of synchronous cultures of Alcaligenes eutrophus H16, prepared by a continuous-flow size selection method. J Gen Microbiol 99: 383–389
Edwards C (1980) Sensitivity of synchronous cultures of Bacillus subtilis to lysozyme. J Gen Microbiol 119:277–279
Edwards C (1981) Methods for investigating the cell cycle. In: The microbial cell cycle. van Nostrand Reinhold, Workingham, pp 5–24
Guerrero R, Mas J, Pedrós-Alió C (1984) Buoyant density changes due to intracellular content of sulfur in Chromatium warmingii and Chromatium vinosum. Arch Microbiol 137:350–356
Helmstetter CE (1969) Methods for studying the microbial division cycle. Methods Microbiol 1:327–361
Koch AL, Blumberg G (1976) Distribution of bacteria in the velocity gradient centrifuge. Biophys J 16:389–405
Kubitschek HE (1968) Constancy of uptake during the cell cycle of Escherichia coli. Biophys J 8:1401–1412
Kubitschek HE (1981) Bilinear cell growth of Escherichia coli. J Bacteriol 148:730–733
Kubitschek HE, Baldwin WW, Graetzer R (1983) Buoyant density constancy during the cell cycle of Escherichia coli. J Bacteriol 155:1027–1032
Lloyd D, John L, Edwards C, Chagla AH (1975) Synchronous cultures of microorganisms: large scale preparation by continuous-flow size selection. J Gen Microbiol 88:153–158
Lloyd D, Poole RK, Edwards SW (1982) The cell division cycle: methods of study. In: The cell division cycle: temporal organisation and control of growth and reproduction. Academic Press, London, 44–93
Martinez-Salas E, Martin JA, Vincente M (1981) Relationship of Escherichia coli density to growth rate and cell age. J Bacteriol 147:97–100
Mas J, Pedros-Alio C, Guerrero R (1985) Mathematical model for determining the effects of intracytoplasmic inclusions on volume and density of microorganisms. J Bacteriol 164:749–756
Mitchison JM (1971) Synchronous cultures. In: The biology of the cell cycle. Cambridge University Press, London, pp 25–57
Nanninga N, Woldringh CK, Koppes LJH (1982) Growth and division of Escherichia coli. In: Nicolini C (ed) Cell growth. Plenum Press, London, pp 225–270
Poole RK (1977) Fluctuations in buoyant density during the cell cycle of Escherichia coli K12: Significance for the preparation of synchronous cultures by age selection. J Gen Microbiol 98:177–186
Pritchard RH (1974) On the growth and form of a bacterial cell. Philos Trans R Soc B 267:303–336
Poindexter JS (1981) The Caulobacters: ubiquitous unusual bacteria. Microbiol Revs 45:123–179
Rosenberger RF, Gover NB, Zaritsky A, Woldringh CL (1978) Surface growth in rod shaped bacteria. J Theor Biol 73:711–721
Sargent M (1979) Surface extension and the cell cycle in prokaryotes. Adv in Microbial Physiol 18:105–176
Trueba FJ, Woldringh CL (1980) Changes in cell diameter during the division cycle of Escherichia coli. J Bacteriol 142:869–878
Woldringh CL, Binnerts JS, Mans A (1981) Variation in Escherichia coli buoyant density measured in Percoll gradients. J Bacteriol 148:58–63
Woldringh CL, Walkendurg JAC, Pas E, Taschner PEM, Huls P, Wienjes FB (1985) Physiological and geometrical conditions for cell division in Escherichia coli. Ann Inst Pasteur 136A:131–138
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Hart, A., Edwards, C. Buoyant density fluctuations during the cell cycle of Bacillus subtilis . Arch. Microbiol. 147, 68–72 (1987). https://doi.org/10.1007/BF00492907
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DOI: https://doi.org/10.1007/BF00492907